30,416 results

1. pQE30 PTBP1 isoform1

   Plasmid

   #108591

   Purpose

   Recombinant His tagged protein production

   Depositor

   Chris Smith

   Article

   Wollerton et al RNA. 2001 Jun;7(6):819-32.

   Insert

   PTBP1 isoform 1 (PTBP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   isoform 1 (isoform c)

   Promoter

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pELxG4

   Plasmid

   #172570

   Purpose

   Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   LuxR-GFPmut3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRKH2

   Plasmid

   #71312

   Purpose

   Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-3c-rhVTN_FL

   Plasmid

   #30223

   Depositor

   James Thomson

   Article

   Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

   Insert

   rhVitronectin without deletions (VTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No deletions

   Promoter

   Available Since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE30-His-T7RNAP

   Plasmid

   #124138

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   T7 RNA polymerase (T7p07 Enterobacteria phage T7)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTG2

   Plasmid

   #149475

   Purpose

   Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   TetR-GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PTetA

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP25-1A(sfGFP)

   Plasmid

   #126972

   Purpose

   Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMALcPP-MS2cp

   Plasmid

   #203351

   Purpose

   Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

   Depositor

   Thomas Cech

   Article

   Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

   Insert

   MS2 coat protein
   

   Use

   Tags

   Hx6-MBP

   Expression

   Bacterial

   Mutation

   V75E and A81G, to prevent capsid assembly

   Promoter

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBR8k-GFPuv

    Plasmid

    #106385

    Purpose

    L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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