30,140 results

1. pFSKm4-ATF1

   Plasmid

   #221133

   Purpose

   Formaldehyde sensor based on the frmR repressor, which expresses yeast alcohol acetyl transferase (ATF1) upon detection

   Depositor

   André Studart

   Article

   Dutto et al Adv Mater. 2024 Dec 10:e2412555. doi: 10

   Insert

   ATF1 (ATF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pm4

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWO-SUMO-3C_14M_zTDP2

   Plasmid

   #200512

   Purpose

   Expression of zebrafish TDP2 recombinant protein

   Depositor

   Antony Oliver

   Insert

   TDP2 (tdp2b Zebrafish)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Sender_plasmid2

   Plasmid

   #140693

   Purpose

   Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

   Depositor

   Matthew Bennett

   Article

   Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

   Insert

   RhlI (rhlI )
   

   Use

   Tags

   ssrA degradation tag (ASV)

   Expression

   Bacterial

   Mutation

   Promoter

   engineered lac promoter (IPTG inducible)

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 gamma-tubulin

   Plasmid

   #101825

   Purpose

   production of gamma-tubulin in bacteria

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Whole protein

   Promoter

   T7

   Available since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCRISPomyces-2

   Plasmid

   #61737

   Purpose

   Streptomyces expression of codon-optimized Cas9 and custom gRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL) and rpsL(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

   Plasmid

   #199390

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi-SUMOEu

   Expression

   Bacterial

   Mutation

   Promoter

   T5-LacO

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET303_sumocodo_GFP_codoopti

   Plasmid

   #210393

   Purpose

   Express SUMO-GFP in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Tags

   SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. eGFP-luciferase-C1

    Plasmid

    #163523

    Purpose

    Expresses both eGFP and firefly luciferase in mammalian cells

    Depositor

    Thomas Weber

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    eGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSAM_Ec

    Plasmid

    #102939

    Purpose

    Transposon mutagenesis and Tn-seq

    Depositor

    Matthew Mulvey

    Article

    Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

    Insert

    mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEGFP-pBAD

    Plasmid

    #54622

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pKM208

    Plasmid

    #13077

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    lacI repressor (lacI E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDisCoTune

    Plasmid

    #173713

    Purpose

    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

    Depositor

    Morten Norholm

    Article

    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

    Inserts

    ERV1 (ERV1 Budding Yeast)
    PDI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSU10-30-50-100

    Plasmid

    #173838

    Purpose

    Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUC01

    Plasmid

    #177204

    Purpose

    chromogenic reporter for gene knockout in Streptomyces

    Depositor

    Wenjun Guan

    Article

    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

    Insert

    SaindC
    

    Use

    Synthetic Biology

    Tags

    NONE

    Expression

    Bacterial

    Mutation

    NONE

    Promoter

    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCasPA

    Plasmid

    #113347

    Purpose

    Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

    Depositor

    Quanjiang Ji

    Article

    Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET mCherry LIC cloning vector (u-mCherry)

    Plasmid

    #29769

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge