30,535 results

1. mTFP1-pBAD

   Plasmid

   #54553

   Purpose

   Localization: Protein Expression Vector , Excitation: 462, Emission: 492

   Depositor

   Michael Davidson, Robert Campbell

   Article

   Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mTFP1

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGD248

   Plasmid

   #219498

   Purpose

   Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HyperNova/pRSETB

   Plasmid

   #221456

   Purpose

   Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDUET-1_VNp-anti-GFP mAb

   Plasmid

   #214955

   Purpose

   Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-GFP mAb heavy chain
   VNp-anti-GFP mAb light chain
   

   Use

   Tags

   VNp6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIntegrase_8

   Plasmid

   #60579

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pReporter_8

   Plasmid

   #60568

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Tight-Puro_MYRF

   Plasmid

   #64843

   Purpose

   A lentiviral vector for the expression of mouse MYRF under doxycyline control.

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   MYRF (Myrf Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVC-Ds-MCS-Ds

   Plasmid

   #102416

   Purpose

   Empty vector with a multiple cloning site flanked by Ds integration elements

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSP3545-dCas9Str

    Plasmid

    #153516

    Purpose

    Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    dCas9Str
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    nisA

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11d-RAN

    Plasmid

    #108919

    Purpose

    Expresses human RAN

    Depositor

    Larry Gerace

    Article

    Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

    Insert

    RAN (RAN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC71174

    Plasmid

    #154067

    Purpose

    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Inserts

    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    

    Use

    Cre/Lox and Synthetic Biology; Golden gate

    Tags

    Expression

    Bacterial and Plant

    Mutation

    The Arabidopsis thaliana U5 small nuclear ribonuc…

    Promoter

    35S promoter, Hordeum vulgare HSP 17 promoter use…

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-3xMBT

    Plasmid

    #46987

    Purpose

    Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    contains amino acids 190–530 of accession NP_0562…

    Promoter

    tac

    Available Since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRF-TetR

    Plasmid

    #49374

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    TetR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a-VHH-tev-mCherry

    Plasmid

    #117752

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET151-HIS-TEV-GST-PP-RPN13

    Plasmid

    #73741

    Purpose

    Expression of hRPN13 in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    6xHIS and GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pRSFDuet-1 ElonginB, ElonginC

    Plasmid

    #230999

    Purpose

    Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Elongin B (Elob Mouse)
    Elongin C (Eloc Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-D2

    Plasmid

    #37470

    Depositor

    Amy Palmer, Roger Tsien

    Article

    Palmer et al Chem Biol. 2006 May;13(5):521-30.

    Insert

    D2 second generation cameleon (calcium sensor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ara

    Available Since

    Oct. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pBbA5c-RFP

    Plasmid

    #35281

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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