34,175 results

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pAX2

Plasmid

#117398

Purpose

allelic exchange vector with GFP merodiploid tracker, temperature-sensitive origin of replication, and TetR-controlled kill switch

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAP114

Plasmid

#120799

Purpose

E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Publication

Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

Insert

Pxyl::mCherryOpt

Expression

Bacterial

Mutation

1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

Promoter

Pxyl

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX6P1-N-FLAG

Plasmid

#119759

Purpose

Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.

Depositor

Andrew Jackson, Martin Reijns

Type

Empty backbone

Tags

FLAG and GST

Expression

Bacterial

Promoter

tac promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pHV738

Plasmid

#117476

Purpose

Expression of NMT1

Depositor

Richard Kahn

Publication

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

NMT1 (NMT1 Human)

Expression

Bacterial

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTP434

Plasmid

#104167

Purpose

Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

Depositor

Dirk Görlich

Publication

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)

Tags

14x Histidine tag and NEDD8 from Brachypodium dis…

Expression

Bacterial

Mutation

3x Cysteines located at bps 493-495, 520-522, and…

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSET-dCas9

Plasmid

#110183

Purpose

Expression of the dCas9 gene in Streptomyces

Depositor

Yinhua Lu

Publication

Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

Insert

dCas9

Use

CRISPR; I

Expression

Bacterial

Mutation

D10A, H840A

Promoter

ermE*p

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Publication

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pIB184-Km

Plasmid

#90195

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Publication

Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

Type

Empty backbone

Tags

None

Expression

Bacterial

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSMART-SOS-GFPuv

Plasmid

#102283

Purpose

Fluorescent sensor of E. coli SOS response

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

cda-GFPuv

Expression

Bacterial

Promoter

cda SOS promoter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBBR8k-GFPuv

Plasmid

#106385

Purpose

L-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

araBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR (in lacZ deficient strain)

Plasmid

#99245

Purpose

For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Publication

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCSW21

Plasmid

#86836

Purpose

bacterial expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Publication

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Expression

Bacterial

Promoter

araBAD

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Publication

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCDB180

Plasmid

#80677

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged protein

Depositor

David Baker

Publication

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Type

Empty backbone

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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