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32,870 results

Showing: 2381 - 2400 of 32870 results

  4. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  9. pLT 653

    Plasmid
    #59996
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    dhc-3 (dhc-3 Nematode)
    klp-16 (klp-16 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59996-plasmid-map-sequence-id-110769

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  10. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  15. pCVL.SSA TLR (Sce)

    Plasmid
    #45576
    Purpose
    Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone
    Depositor
    Andrew Scharenberg
    Article
    Kuhar et al Nucleic Acids Res. 2013 Oct 10.
    Inserts
    5' iRFP arm
    eGFP with I-Sce I TS
    +3 mCherry
    3' iRFP arm
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Mutation
    embedded I-Sce I TS from 163-185bp, truncated 25 …
    Promoter
    SFFV
    Available Since
    Nov. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    45576-plasmid-map-sequence-id-67850

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  20. AURKB

    Plasmid
    #39119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AURKB (AURKB Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2381 - 2400 of 32870 results