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pD454-SR mouse Alpha-synuclein

Plasmid

#89075

Purpose

bacterial expression of mouse Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Mouse)

Expression

Bacterial

Promoter

T7

Available Since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pQLinkN

Plasmid

#13670

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Article

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-eyfp

Plasmid

#65031

Purpose

GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAraH6HATT

Plasmid

#63901

Purpose

For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

Depositor

Carlos Barbas

Article

Barbas et al Methods, A companion to Methods in Enzym

Insert

TT Fab

Tags

6xHis and HA tag

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBSTNAV

Plasmid

#45801

Depositor

Luc Ponchon

Article

Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15

Plasmid

#176212

Purpose

Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMU131

Plasmid

#66811

Purpose

Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI

Depositor

Lee Lynd

Article

Shaw et al Appl Environ Microbiol. 2010 Jul;76(14):

Type

Empty backbone

Expression

Bacterial

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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5KO9 (HMCES)

Plasmid

#101877

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HMCES (HMCES Human)

Tags

6x His (modified C-term tag is AENLYFQSHHHHHH)

Expression

Bacterial

Promoter

T7

Available Since

April 30, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pBTR1

Plasmid

#22468

Depositor

Gary Pielak

Article

Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

Insert

cytochrome c (CYCS Human)

Expression

Bacterial

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pBAD-ApplePy1Lowest

Plasmid

#239057

Purpose

Bacterial expression of lowest affinity red fluorescent pyruvate biosensor ApplePy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

ApplePy1Lowest

Expression

Bacterial

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRMT1-Halo

Plasmid

#175334

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRMT1 (PRMT1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10

Plasmid

#24551

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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