30,577 results

1. p46Cpf1-OP2

   Plasmid

   #98592

   Purpose

   Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.

   Depositor

   Qiong Wu

   Article

   Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E. coli

   Promoter

   araBAD

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAPK1

   Plasmid

   #73248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn7-156

   Plasmid

   #118570

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mCardinal
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HA-human OCRL

   Plasmid

   #22207

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Oculocerebrorenal syndrome of Lowe (OCRL Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-HisRS-His

   Plasmid

   #124111

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Histidyl-tRNA ligase (hisS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRD4

   Plasmid

   #38943

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWUR790

   Plasmid

   #101723

   Purpose

   Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

   Depositor

   John van der Oost

   Article

   Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   PfAgo
   

   Use

   Tags

   Strep(II)-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promotor

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQE30-His-IF2

   Plasmid

   #124124

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 2 (infB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTurquoise2-pBAD

    Plasmid

    #54844

    Purpose

    Localization: Protein Expression Vector , Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    6x His, T7 tag, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLR16-Phes*

    Plasmid

    #98783

    Purpose

    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

    Depositor

    Anat Herskovits

    Article

    Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A309G

    Promoter

    Available Since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTOX6

    Plasmid

    #127451

    Purpose

    Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    tse2 toxin
    amilCP chromoprotein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUltra-chPheRS

    Plasmid

    #182257

    Purpose

    Genetic Incorporation of DOPA

    Depositor

    Han Xiao

    Article

    Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

    Inserts

    chPheRS2 for DOPA
    chPheT for DOPA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM-41-HsPABPC1_J

    Plasmid

    #146644

    Purpose

    Bacterial Expression of HsPABPc1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsPABPc1 (PABPC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW-LIC-N8C_Fab3a

    Plasmid

    #203689

    Purpose

    Expresses N8C_Fab3a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab3a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-CysRS-His

    Plasmid

    #124107

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Cysteinyl-tRNA ligase (cysS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBad-HisB-RRvT

    Plasmid

    #87364

    Purpose

    Expresses RRvT, a tandem heterodimer red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    RRvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad (Arabinose)

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pS2513·PHP

    Plasmid

    #122590

    Purpose

    Constitutive expressed pHiorine2 for pH quan. in gram-negative cells

    Depositor

    Pablo Ivan Nikel

    Article

    Arce-Rodriguez et al Microb Biotechnol. 2019 Jun 4. doi: 10.1

    Insert

    phIorine2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pem7

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. His6-EGFP-PH-PLCD1

    Plasmid

    #183675

    Purpose

    PtdIns(4,5)P2 biosensor for purification from bacteria

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    PLCD1 (PLCD1 Human)
    

    Use

    Tags

    His6-EGFP

    Expression

    Bacterial

    Mutation

    Amino acids 11-140

    Promoter

    T7

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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