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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Publication

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Tags

pEGFP

Expression

Bacterial and Mammalian

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Publication

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Traptavidin

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFPV-mCherry

Plasmid

#20956

Depositor

Olivia Steele-Mortimer

Publication

Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

Insert

mCherry

Expression

Bacterial

Available since

June 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Publication

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor Q63L (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Publication

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Publication

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3113 GST-p53

Plasmid

#10852

Depositor

Peter Howley

Publication

Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC019 - LwCas13a from Leptotrichia wadei

Plasmid

#91909

Purpose

Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a and guide expression cassette

Use

CRISPR

Expression

Bacterial

Available since

Oct. 4, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Publication

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Publication

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-(-30)GFP

Plasmid

#62936

Purpose

Expression of 6xHis-(-30)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Publication

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET21b(+)-Is-PETase-W159H-S238F

Plasmid

#112203

Purpose

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

Depositor

Gregg Beckham, Christopher Johnson

Publication

Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

Insert

PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12

Tags

6XHIS

Expression

Bacterial

Mutation

W159H, S238F

Promoter

N/A

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDest-566

Plasmid

#11517

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway Cloning

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Publication

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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