31,059 results

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pEVOL-pylT-AznLRS

Plasmid

#172482

Purpose

For E.Coli expression, 306L309L348I384F411W

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

Insert

AznLRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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GST-GGA3-VHS-GAT

Plasmid

#173719

Purpose

VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

Depositor

Juan Bonifacino

Article

Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

Insert

GGA3 (GGA3, GGA2, GGA1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMega-MaPylRS

Plasmid

#200225

Purpose

The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

Depositor

Alanna Schepartz

Article

Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

Inserts

Pyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA

Tags

none

Expression

Bacterial

Mutation

none

Promoter

proK-lacO and tacI

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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His6-SUMO-pET28a-EfaCas1

Plasmid

#112793

Purpose

To express enterococcus faecalis Cas1 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas1

Tags

His6-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPdR

Plasmid

#85083

Purpose

Expresses putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin reductase

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pTW411d

Plasmid

#246585

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, NtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pYFJ84

Plasmid

#227644

Purpose

Vibrio harveyi acyl-ACP synthetase

Depositor

John Cronan

Article

Jiang et al Biochemistry. 2006 Aug 22;45(33):10008-1

Insert

Acyl-Acyl carrier protein synthetase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-Leup

Plasmid

#213154

Purpose

Expresses the leupeptin protease inhibitor pathway for bolstered production of heterologously expressed proteins.

Depositor

Jason Crawford

Article

Li et al J Nat Prod. 2021 Oct 22;84(10):2692-2699

Insert

Leupeptin Protease Inhibitor Pathway

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pQE80l BCB

Plasmid

#196472

Purpose

Protein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher

Depositor

Fei Sun

Article

Wang et al Proc Natl Acad Sci U S A. 2017 Jun 6;114

Insert

BCB

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mECitrine

Plasmid

#105151

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

kanMX6-P3nmt1-mECitrine

Tags

3nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

3nmt1

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBbE8a-RFP

Plasmid

#35268

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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