29,729 results

1. pMYCO1_SpdCas9_pmcDA1

   Plasmid

   #204615

   Purpose

   CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1_mNeongreen-Etanercept

   Plasmid

   #182420

   Purpose

   Bacterial expression of mNeongreen-Etanercept fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-Etanercept (TNFRSF1B Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS PKC theta

   Plasmid

   #8426

   Depositor

   Frederic Mushinski

   Article

   Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

   Insert

   Mouse Protein Kinase C theta (Prkcq Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pMBEC2

   Plasmid

   #187596

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p15aC-GFP

   Plasmid

   #107862

   Purpose

   Control plasmid expressing GFP

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   GFP
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23b-NTF2

   Plasmid

   #108918

   Purpose

   Expresses NTF2

   Depositor

   Larry Gerace

   Article

   Cassany et al Methods Mol Biol. 2009;464:181-205. doi:

   Insert

   NTF2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. WIW_Scaffold2

   Plasmid

   #205574

   Purpose

   Nanobody that inhibits seeding of tau fibrils

   Depositor

   David Eisenberg

   Article

   Abskharon et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   WIW_Scaffold2
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD112

   Plasmid

   #119893

   Purpose

   pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTOX1

   Plasmid

   #127446

   Purpose

   Encodes YhaV toxin. CAM-R

   Depositor

   Matthew Waldor

   Article

   Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

   Insert

   yhaV toxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRha

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Paxillin

    Plasmid

    #186142

    Purpose

    For expresseion of recombinant paxillin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Paxillin C1997A (silent mutation for cloning)

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR562

    Plasmid

    #207886

    Purpose

    Creation of Level 0 entry clones

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    amilCP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTXB1-pA-M.EcoGII

    Plasmid

    #192873

    Purpose

    pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

    Depositor

    Chong Tang

    Article

    Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

    Insert

    pA-M.EcoGII
    

    Use

    Tags

    3X Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-PIPK1 gamma 90

    Plasmid

    #22299

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid

    #182395

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & Leucine Zi…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-45b-mEGFP-HMGB1-WT

    Plasmid

    #194543

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO3

    Plasmid

    #52260

    Purpose

    Bacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Inserts

    SUMO-3 (SUMO3 Human)
    UBE2l (UBE2I Human)
    SAE-2 (UBA2 Human)
    SAE-1 (SAE1 Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    C-terminal gly-gly

    Promoter

    T7 and rbs

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6CC8 (MBD3)

    Plasmid

    #119959

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Tags

    GSTtag, 6xHis and Thrombin

    Expression

    Bacterial

    Mutation

    MBD domain (contains residues 1-71)

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pLRD11

    Plasmid

    #208263

    Purpose

    Production of sclareol in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    abCAS
    ssSS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    abCAS(D537A)

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. VHL-pGex2TK

    Plasmid

    #20790

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    VHL (VHL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    VHL wt cDNA, no mutations

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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