30,744 results

1. GST-3C-FIP200-GFP

   Plasmid

   #187832

   Purpose

   Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   FIP200 (RB1CC1 Human)
   

   Tags

   GST and mGFP

   Expression

   Bacterial and Insect

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

   Plasmid

   #74102

   Purpose

   plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

   Depositor

   Ben Szaro

   Article

   Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

   Inserts

   Xenopus laevis beta-tubulin gene promoter region and 5'UTR
   Green Lantern Green Fluorescent Protein
   Rabbit beta 1-globin gene 3'UTR
   

   Expression

   Bacterial

   Available Since

   May 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-cerebody

   Plasmid

   #236738

   Purpose

   Protein overexpression of cerebody

   Depositor

   Christina Woo

   Article

   Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

   Insert

   cerebody
   

   Tags

   His, HA, FLAG

   Expression

   Bacterial

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p27-mVenus reporter (PB)

   Plasmid

   #190731

   Purpose

   The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   mVenus
   p27
   

   Expression

   Bacterial and Mammalian

   Mutation

   p27-F62A-F64A

   Promoter

   EF1α

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-GST-BcPC-PLC

   Plasmid

   #224255

   Purpose

   Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

   Depositor

   Chiaki Murakami

   Article

   Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

   Insert

   Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)
   

   Tags

   GST and HRV-3C (LEVLFQGP) site

   Expression

   Bacterial

   Mutation

   The gene of Bacillus cereus ATCC 14579 was used f…

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR1051

   Plasmid

   #208739

   Purpose

   Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PZwf
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PZwf

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR1057

   Plasmid

   #208742

   Purpose

   Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

   Depositor

   Lauren Andrews

   Article

   Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

   Insert

   PPgk
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PPgk

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_CRK_SH3

   Plasmid

   #178472

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CRK_SH3 (CRK Human, Synthetic)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)

   Plasmid

   #78507

   Purpose

   Construct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labeling

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   Gads SH3 SH2 SH3 domain (GRAP2 Human)
   

   Tags

   GST followed by a prescission protease claevage s…

   Expression

   Bacterial

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCL147

    Plasmid

    #134454

    Purpose

    TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.

    Depositor

    Thomas Bernhardt

    Article

    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

    Insert

    linker-mScarlet-I for C-terminal fusion
    

    Expression

    Bacterial

    Promoter

    plac

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTNS2

    Plasmid

    #87802

    Purpose

    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

    Depositor

    Yu-Zhu Zhang

    Article

    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

    Type

    Empty backbone

    Tags

    6xHis and Strep II

    Expression

    Bacterial

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL-rpb1-E1103G

    Plasmid

    #10989

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-E1103G (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 3308 A to G in the RPB1 ORF resulting in…

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pGPA1

    Plasmid

    #115476

    Purpose

    Generation of mariner-based transposon mutant libraries in Enterococcus faecium

    Depositor

    Willem van Schaik

    Article

    Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-Sox2

    Plasmid

    #16353

    Depositor

    James Thomson

    Article

    Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

    Insert

    Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE140

    Plasmid

    #118492

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Expression

    Bacterial

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNCST-AausFP1

    Plasmid

    #129499

    Purpose

    Expresses AausFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    AausFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9(D10A)

    Plasmid

    #74495

    Purpose

    Nicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10A

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

    Insert

    Cas9 D10A
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    pTeto

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLibacceptorV2

    Plasmid

    #106250

    Purpose

    Recombination-mediated cassette exchange with target genomic loci

    Depositor

    Sriram Kosuri

    Article

    Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only