30,260 results

1. pET LIC cloning vector (2A-T)

   Plasmid

   #29665

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdestDD04Neo

   Plasmid

   #26386

   Depositor

   Denis Dupuy

   Article

   Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDE43-MCSq17

   Plasmid

   #27333

   Depositor

   Dirk Schnappinger

   Article

   Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

   Insert

   qtag17
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLUK

   Plasmid

   #64181

   Purpose

   harbours marker genes HIS3, LEU2, URA3 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FKBP8

   Plasmid

   #25609

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   FKBP8 (FKBP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   FKBP-type PPIase Domain, residues 90-205

   Promoter

   Available since

   Aug. 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. WT FL USP7

   Plasmid

   #227214

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7-promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-BoNT/X

   Plasmid

   #168017

   Purpose

   Expresses BoNT/X-LC(1-439)-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   BoNT/X-LC(1-439)-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLG027 (qatABCD)

   Plasmid

   #157905

   Purpose

   Expresses the qatABCD defense system

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2020 Aug 28;369(6507):1077-1084

   Insert

   qatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFP.E227

    Plasmid

    #138249

    Purpose

    Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    mCherry
    Terminator
    mTagBFP
    Terminator
    gfp
    Terminator
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    P17_up1691, P20_992, and iP16_3622

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

    Plasmid

    #117910

    Purpose

    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

    Depositor

    Kai Johnsson

    Article

    Yu et al Science. 2018 Sep 14;361(6407):1122-1126

    Insert

    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    R44A on eDHFR

    Promoter

    T7

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLPO2

    Plasmid

    #209976

    Purpose

    Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (SH71_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid

    #117006

    Purpose

    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLG166

    Plasmid

    #124366

    Purpose

    Gfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilis

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    gfpmut2 (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppen

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mu-FLT3Lv1-pTRIP

    Plasmid

    #163519

    Purpose

    Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine-FLT3L variant 1 (Flt3l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-SUMO1

    Plasmid

    #185637

    Purpose

    expression of GST-tagged SUMO1 in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    small ubiquitin-like modifier 1 (SUMO1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-hphMX6-81nmt1

    Plasmid

    #105164

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-81nmt1
    

    Use

    Tags

    81nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    81nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-hphMX6-41nmt1

    Plasmid

    #105163

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

    Insert

    hphMX6-41nmt1
    

    Use

    Tags

    41nmt1

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    41nmt1

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30-2-PSAT1

    Plasmid

    #83908

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    phosphoserine aminotransferase 1 (PSAT1 Human)
    

    Use

    Tags

    6x His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge