32,395 results

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pMAL-c5X-GLP-1

Plasmid

#66997

Purpose

Expresses malE-GLP-1 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-GLP-1

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pWUR 1+2 tetO mut89

Plasmid

#80102

Purpose

Encodes a set of wild-type Cas1+2 and a mutant set of Cas1+2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
Cas1 (cas1 )
Cas2 (cas2 )

Expression

Bacterial

Mutation

Q24H, P202Q, G241D, E276D, L297Q

Available Since

Dec. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MAGEA4

Plasmid

#39001

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MAGEA4 (MAGEA4 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

Plasmid

#181792

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Ran(Q69L)

Plasmid

#42048

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Ran (RAN Human)

Tags

His

Expression

Bacterial

Mutation

Q69L

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pTW386c

Plasmid

#237321

Purpose

expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pQE81L-KOFP7

Plasmid

#160330

Purpose

This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.

Depositor

Sang Taek Jung

Article

Ko et al J Agric Food Chem. 2020 May 27;68(21):58

Insert

KOFP7

Tags

6xHis tag

Expression

Bacterial

Promoter

T5

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Expression

Bacterial

Promoter

Veillonella parvula SKV38 mdh promoter

Available Since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28b(+)_rPD-L1nb

Plasmid

#226715

Purpose

Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag and Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTKEI-Tre-C04

Plasmid

#79754

Purpose

Single fluorescent protein biosensor for trehalose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Tre-C04

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMG046 pETARA-CK1α-His

Plasmid

#154074

Purpose

Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSopBC460S

Plasmid

#112514

Purpose

PsopB driven sopB (catalytically inactive) and sigE

Depositor

Olivia Steele-Mortimer

Article

Finn et al PLoS Pathog. 2017 Apr 20;13(4):e1006354.

Insert

sopB (catalytically inactive)/sigE operon

Expression

Bacterial

Mutation

SopB C460S

Promoter

PsopB

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET-NtC60αC20NtLC60β

Plasmid

#160877

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pDF0039 DisCas7-11 only expression with CRISPR array

Plasmid

#172500

Purpose

Encodes the DisCas7-11 protein only for bacterial expression

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 only expression with CRISPR array

Expression

Bacterial

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA

Plasmid

#108236

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter

Expression

Bacterial

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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