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Plasmid#160818PurposeaTc-responsive single-layered circuitDepositorInsertPJ23117-30tetR-t-Ptet2-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23117, Ptet2Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOmpF
Plasmid#42606DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP5
Plasmid#61750Purposeexpress N-terminally GST tagged human USP5DepositorInsertUSP5 (USP5 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLZL2386
Plasmid#48682PurposepET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)DepositorInsertPFV Integrase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
SRPK1 (SRPK1A-c100)
Plasmid#98246PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSRPK1A (SRPK1 Human)
UseTagsHis-6-TEVExpressionBacterialMutationPromoterT7Available sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRC_HUMAN_D0
Plasmid#79700PurposeThis plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSRC (SRC Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b His-p97
Plasmid#169020PurposeExpresses His-p97 in bacteriaDepositorInsertp97
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP3653
Plasmid#222348PurposeExpression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His-SCov2-nsp7
Plasmid#154757PurposeExpression of SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11a_PTK2b_Kinase
Plasmid#224242PurposeExpression of human PTK2b Kinase domain in E. coliDepositorInsertptk2b_kinase (PTK2B Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
UseTagsNone.ExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHuji
Plasmid#61555PurposeRed fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHuji
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
UseTagsExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneUseTagsHis8-SumoExpressionBacterialMutationPromoterT7Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
RHOAA-c033
Plasmid#205116PurposeRHOA protein expressionDepositorInsertRHOA (RHOA Human)
UseTags6His-TEVExpressionBacterialMutationWTPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-Phi29
Plasmid#207456Purposein vitro transcription of mRNADepositorInsertMCP-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG029 (mzaABCDE)
Plasmid#157907PurposeExpresses the mzaABCDE defense systemDepositorInsertmzaABCDE (5-gene system containing a MutL homolog)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT203
Plasmid#134880PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only