31,059 results
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Plasmid#71753PurposeU+36GFP-BirA for expression in E.ColiDepositorInsertU_(pos36GFP)_BirA_6XHis_LPETG
Tags6XHisExpressionBacterialAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS413-GAL1-luc*(-SKL)
Plasmid#40234DepositorInsertluc*(-SKL)
ExpressionBacterialPromoterGAL1Available SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp1
Plasmid#156465PurposeBacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tagDepositorInsertNsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-RNMT
Plasmid#112709PurposeExpresses N-terminally GST-tagged human RNMT in bacterial cellsDepositorAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLRD04
Plasmid#208258PurposeProduction of ent-kaurene in E. coli MEV20DepositorInsertstHMGR
ptmT2
blr2150
ExpressionBacterialAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv2
Plasmid#188604PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBZCas13b-HEPN
Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIRT6
Plasmid#41564PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeExpressionBacterial and MammalianPromoterNoneAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28-iLOV-Rsn2
Plasmid#61687Purposebacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOVDepositorInsertRanaspumin-2
TagsHis and iLOVExpressionBacterialPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFAB808
Plasmid#47853PurposeBIOFAB reporter plasmid for measuring rrnB T1 termination efficiencyDepositorInsertrrnB T1 terminator
UseSynthetic BiologyExpressionBacterialPromoterpLTetO8Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPO717
Plasmid#195302PurposepBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.DepositorInsertMcCAST Cascade and lacZ spacer 5 in native array
ExpressionBacterialPromoterArabinose inducible arapBADAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-130
Plasmid#118516PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAKgfp2
Plasmid#14077DepositorInsertlacIq repressor gene
ExpressionBacterialAvailable SinceMarch 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
EC71173
Plasmid#154061PurposeLevel 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-CreDepositorInsertCre recombinase
ExpressionBacterialMutationIntroduction of U5 intron sequences, to prevent p…PromoterHvHSP17Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PUM1_PUM_pGEX
Plasmid#135129PurposeRecombinant expression of PUM1 with dual-affinity tagDepositorAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPR2
Plasmid#209980PurposeContains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmidsDepositorInsertresistance cassette (cat)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPE1
Plasmid#209981PurposeContains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (pMB1_ampR_B0017)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPE2
Plasmid#209982PurposeContains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmidsDepositorInsertE. coli shuttle part (ampR_B0017)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC54
Plasmid#209983PurposeContains Level 0* Part: 5' Connector (5C_07_5C1C) for the construction of Level 2 plasmidsDepositorInsert5' Connector (5C_07_5C1C)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPC33
Plasmid#209984PurposeContains Level 0* Part: 3' Connector (3C_08_3C3) for the construction of Level 2 plasmidsDepositorInsert3' Connector (3C_08_3C3)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPE3
Plasmid#209985PurposeContains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmidsDepositorInsertE. coli shuttle part (pMB1_cat)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPR3
Plasmid#209986PurposeContains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmidsDepositorInsertresistance cassette (ermBL)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPO5
Plasmid#209987PurposeContains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmidsDepositorInsertreplication origin (256_rep)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP3
Plasmid#209965PurposeContains Level 0 Part: constitutive Promoter (P25) for the construction of Level 1 plasmidsDepositorInsertPromoter (P25)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP5
Plasmid#209967PurposeContains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmidsDepositorInsertPromoter (P_sppQ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP7
Plasmid#209969PurposeContains Level 0 Part: autoinducible Promoter (PfabZ) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_fabZ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP8
Plasmid#209970PurposeContains Level 0 Part: autoinducible Promoter (PgadB) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_gadB)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP9
Plasmid#209971PurposeContains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmidsDepositorInsertPromoter (P_ldhD)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPS2
Plasmid#209972PurposeContains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmidsDepositorInsertRBS (Lp)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPT2
Plasmid#209974PurposeContains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmidsDepositorInsertTerminator (T_pepN)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPR1
Plasmid#209979PurposeContains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmidsDepositorInsertresistance cassette (ermBL)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLP3
Plasmid#209958PurposeEntry vector to store Level 0 broad-host range L. plantarum replication origin partsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP1
Plasmid#209963PurposeContains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmidsDepositorInsertPromoter (mock)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLPP2
Plasmid#209964PurposeContains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmidsDepositorInsertPromoter (P48)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pet15b_ack
Plasmid#220229PurposeBacterial expression of AcK in E. coli BL21_DE3 (pLys) or similarDepositorAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC31
Plasmid#209996PurposeContains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC32
Plasmid#209997PurposeContains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmidsDepositorInsert3' Connector (3C5CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC52
Plasmid#209991PurposeContains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C2SN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPC51
Plasmid#209990PurposeContains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmidsDepositorInsert5' Connector (5C1CSN)
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1_LIM
Plasmid#109780PurposeProtein expression and purification of LASP1_LIMDepositorInsertLASP1_LIM (LASP1 Human)
ExpressionBacterialAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbB9a-GFP
Plasmid#126112PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23113Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PhoCl
Plasmid#87688PurposeExpress PhoCl in E. coliDepositorInsertPhoCl
Tags6xHisExpressionBacterialAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PTPN5
Plasmid#39169PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Mms2
Plasmid#18893DepositorAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in LT)
Plasmid#70033PurposepUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding regionDepositorInsertTSPyV full genome, linearized in Large T-antigen
UseCloning vectorExpressionBacterialAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only