32,671 results
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Plasmid#42478PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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pEMF037
Plasmid#157634PurposeBatis maritima MHTDepositorInsertMHT
ExpressionBacterialAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK066
Plasmid#65173PurposeEncodes tTDH1 as a Type 4b part to be used in the Dueber YTK systemDepositorInserttTDH1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
ExpressionBacterialPromoterE . coli insulated waaH gene promoter, low phosp…Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
VV020: WT Arch-3 - eGFP in pET28b
Plasmid#58488PurposeExpresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coliDepositorInsertArchaerhodopsin-3
TagseGFPExpressionBacterialPromoterT7Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-CoilR-Cys
Plasmid#181989PurposeUsed to label proteins with the CoilE genetically encoded tagDepositorInsertCoilR-Cys
TagsHis-TagExpressionBacterialAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIRT5.1
Plasmid#13738DepositorAvailable SinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
ELK1_pet28a
Plasmid#131647PurposeBacterial expression of ETS gene ELK1DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PDCD6IP_ALIX V-domain
Plasmid#178483PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPDCD6IP_ALIX V-domain (PDCD6IP Human, Synthetic)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGC4593
Plasmid#110206PurposeAccessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)DepositorInsertsTEV protease
aaRS
ExpressionBacterialPromoterPbad and PtetAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Src(WT)-mCherry
Plasmid#87356PurposePI(WT): Photo-inhibitable/blue light sensitive ; WT: wild typeDepositorAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
ExpressionBacterialPromoterPT7 and PproKAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
RS35075_pMQ30_KO_Kan
Plasmid#185390PurposeNon-replicating vector used to create markerless deletion of RR42_RS35075 in C. basilensis 4G11DepositorInsertsRR42_RS35075 upstream flanking homology region
RR42_RS35075 downstream flanking homology region
nptII kanamycin resistance marker
ExpressionBacterialAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRII-1.1-1.10a
Plasmid#69589PurposeOT-II TCR a chain cDNA in pCRIIDepositorInsertTCR alpha clone 1.1 (Tcra Mouse)
ExpressionBacterialAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorInsertPropionate inducible promoter
UseSynthetic BiologyExpressionBacterialPromoterprpAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
TagsTRX-HisExpressionBacterialPromoterT7Available SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJGL002
Plasmid#180606PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasXDepositorInsertPlmCasX
UseCRISPRTags10xHis and MBPExpressionBacterialPromoterT7Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-D77
Plasmid#18896DepositorInsertUbiquitin-D77 (UBC Human)
ExpressionBacterialMutationAspartate residue is added to the C-terminus of t…Available SinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
ExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable SinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-His6
Plasmid#51532PurposeExpress His6-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK90
Plasmid#13323DepositorInsertgyrA
ExpressionBacterialAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
Tags6X Histidine Tag, TEV cleavage siteExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-EFTs
Plasmid#31173DepositorInsertE. coli elongation factor Ts
TagsHisExpressionBacterialAvailable SinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109Hg-gfp
Plasmid#123148PurposeA mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerTAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
TagsmClover3ExpressionBacterial and YeastPromoterSpH2A/Bpro (divergent)Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBluescript GPX-1
Plasmid#13038DepositorInsertGPX-1 (GPX1 Human)
ExpressionBacterialMutationAdditional GCG upstream of codon 11. Codon 92 is …Available SinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
DNMT3A
Plasmid#25328PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SRF WT pGEX4T
Plasmid#121100PurposeExpression of SRF as a GST fusion proteinDepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
ExpressionBacterialMutationWTPromoterT7Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKB224
Plasmid#170014PurposeEncodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
NC
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC37
Plasmid#111618PurposeFluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosaDepositorInsertPpqsA-gfp(ASV)
ExpressionBacterialAvailable SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJDC182
Plasmid#60854PurposeExpresses firefly luciferaseDepositorInsertFirefly luciferase (optimized for Mycobacteria)
ExpressionBacterialMutationcodon optimized for MycobacteriaPromoterhsp60Available SinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJT195
Plasmid#202294PurposeType IIS cloning plasmid for LPattB7 integrations w/ OriTDepositorInsertsfGFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#128280Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
ExpressionBacterialAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
Tags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available SinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialPromoterT7Available SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRA1EGFP
Plasmid#193791PurposeExpresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selectionDepositorInsertEGFP
ExpressionBacterialPromoterPR promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-ald*-alaE
Plasmid#134939PurposeLow phosphate inducible alanine dehydrogenase and alanine exporterDepositorInsertalanine dehydrogenase and L-alanine exporter
ExpressionBacterialPromoteryibDAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
B1-3
Plasmid#111465Purposetranslational release factor 3DepositorInserttranslational release factor 3
Tags6xHisExpressionBacterialAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Caspase-6
Plasmid#100102PurposeBacterial expression of Caspase-6DepositorAvailable SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHS1928
Plasmid#205967PurposeJAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)DepositorInsertCas8-HNH all proteins, with His-tagged Cas8
Tags14xHis on Cas8ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
ExpressionBacterialMutationLysine 622 was replaced by AlanineAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3 I0500 LuxI
Plasmid#107965PurposeArabinose-inducible 3OC6HSL sender plasmidDepositorInsertluxI
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS xenopus Islet1 (DM#219)
Plasmid#15015DepositorInsertislet1
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDx_mRuby3-SYFP2
Plasmid#189758PurposeDual expression vector for bacteria and mammalian cells producing mRuby3-SYFP2 fusion protein for FRET analysisDepositorInsertmRuby3-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p53 R175H
Plasmid#39480DepositorAvailable SinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-RIPK3
Plasmid#78827PurposeTo purify GST-tagged recombinant RIPK3 proteinDepositorAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only