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Plasmid#69802PurposeExpression of H. pylori J99 methylthioadenosine nucleosidaseDepositorInsertMethylthioadenosine nucleosidase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSP578
Plasmid#98808PurposeExpression VectorDepositorInsertpET28 MBP-HNLYFQS-GST
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP808
Plasmid#98812PurposeExpression VectorDepositorInsertpET28 MBP-HNLYGHS-GST
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCoofy21
Plasmid#43979DepositorTypeEmpty backboneUseTagsHis10-OneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pPhlF-P2
Plasmid#74686PurposePhlF-P2 gateDepositorInsertPTac-RBSP2-PhlF-PPhlF-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS CRX (CC#4)
Plasmid#15127DepositorInsertCRX (Crx Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse PEA3 [TJ#200]
Plasmid#16346DepositorInsertPEA3 (Etv4 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5
Plasmid#157804PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1DepositorInserteGFP-bromo5
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLO 4.01
Plasmid#121495Purposeengineered Mu transposonDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialMutationPromoterPotefAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
4A3.SU
Plasmid#197675PurposeExpresses P1 S tail fibre and U chaperoneDepositorInsertsS
U
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.S'U'
Plasmid#197676PurposeExpresses P1 S' tail fibre and U' chaperoneDepositorInsertsS'
U'
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNX6-PX (27-171)
Plasmid#119087PurposeBacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)DepositorInsertSNX6-PX (29-170) (SNX6 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW107-MbCas12a-33362
Plasmid#115654PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G
Plasmid#47646PurposepAC-EsaR with a a272g mutation in esaRDepositorInsertesaR-D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to GlycinePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sap-SH2
Plasmid#46477DepositorInsertSap (SH2D1A Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 4-128PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Pdx1-RFP puro (DM#235)
Plasmid#15085DepositorInsertPdx1 promoter (Pdx1 Mouse)
UseTagsRFPExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRex
Plasmid#31349DepositorInsertThermus aquaticus Redox Sensing Repressor
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Sensor_32B_LacZ
Plasmid#75007PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_32B_LacZ
UseTagsLacZExpressionBacterialMutationPromoterT7Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-FUS 465-526
Plasmid#44983DepositorInsertFUS (465-526aa) (FUS Human)
UseTagsGSTExpressionBacterialMutationcontains amino acids 465-526PromotertacAvailable sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2
Plasmid#13385DepositorInsertSumo2 (SUMO2 Human)
UseTagsEGFP and GSTExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAV105
Plasmid#63195PurposepRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SRSF9_2xRRM_pGEX
Plasmid#135120PurposeRecombinant expression of SRSF9 with dual-affinity tagDepositorInsertSRSF9 (SRSF9 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
UseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
B1 Integrin-GFP
Plasmid#186138PurposeFor expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP and mEGFPExpressionBacterialMutationPromoterLacAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop B
Plasmid#171780PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-DogTag Loop B
UseTagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
STK3_HUMAN_D0
Plasmid#79689PurposeThis plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertSTK3 (STK3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pHoran4
Plasmid#61558PurposeOrange fluorescent protein based pH indicator in bacterial expression vectorDepositorInsertpHoran4
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
UIMLx2
Plasmid#174888PurposeUIMLx2 ubiquitin probe for K48 ubiquitylated proteinsDepositorInsertMet4 UIML
UseTagsAvi-6xHis-Smt3ExpressionBacterialMutationPromoterAvailable sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEN25-Ub Hs
Plasmid#83232PurposepHis-par2-Ub HsDepositorInsertUbiqutin (UBC Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP019
Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
UseTagsExpressionBacterialMutationH134R mutationPromoteraraBADAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-17b_Unc104-KIF5B-HALO
Plasmid#169996PurposeUltra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labelingDepositorInsertKinesin-like protein Unc-104 fused with KIF5B
UseTagsHALO-tag, 6xHISExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_v2
Plasmid#183692PurposeBacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)DepositorInsertER Halo(K73T)
UseTagsExpressionBacterialMutationK73T in HalotagPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_173
Plasmid#39830DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP120
Plasmid#71732Purposeinducible expression of TRP120 in E. coliDepositorInsertTRP120
UseTags6xHIS and ThioredoxinExpressionBacterialMutationPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET23a RanGAP
Plasmid#13387DepositorInsertRanGAP (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
UseTagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOGG216
Plasmid#114002PurposeLevel 2 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
DARS_pNIC-Bio3
Plasmid#153048PurposeExpression construct for producing Avi tagged Glycyl-tRNA synthetaseDepositorInsertDARS (DARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP1171
Plasmid#119253Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1170
Plasmid#119252Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZcsx27
Plasmid#89900PurposeExpresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx27
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHIP2-SH2
Plasmid#46493DepositorInsertSHIP2
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 8-131PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only