32,157 results

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pML433

Plasmid

#177190

Purpose

Expresses recombinant HyPro enzyme in E. coli

Depositor

Eugene Makeyev

Article

Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

Insert

HyPro enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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mu-FLT3Lv1-pTRIP

Plasmid

#163519

Purpose

Used to construct lentivirus to express murine FLT3 ligand (variant-1) in mammalian cells

Depositor

Thomas Weber

Insert

murine-FLT3L variant 1 (Flt3l Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPPi-30m

Plasmid

#13556

Depositor

Wolf Frommer

Article

Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

Insert

FLIPPi T22A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

T22A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pH6HTC_NAP1L1-Halo

Plasmid

#175342

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

NAP1L1 (NAP1L1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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FH

Plasmid

#38978

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

FH (FH Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pKD184

Plasmid

#90953

Purpose

Expresses ThsR under the PltetO-1 promoter, sfGFP under PphsA, and mCherry constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Inserts

Thiosulfate response regulator
Superfolder GFP
mCherry

Expression

Bacterial

Promoter

Bba_J23114, PltetO-1, and PphsA

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

GST

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pDNA2.0-6H-TEV-KRAS G12D

Plasmid

#159439

Purpose

Expresses KRAS G12D in E.coli

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS G12D (KRAS Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_35bpLinker

Plasmid

#157790

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_35bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCNA_PCNA

Plasmid

#110022

Purpose

Protein expression and purification of PCNA_PCNA

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCNA_PCNA (PCNA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mRuby3

Plasmid

#244106

Purpose

Bacterial expression of green glucose sensor with inert mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Tags

mRuby3

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Dcp1-EGFP

Plasmid

#201416

Purpose

Bacterial protein expression of Dcp1

Depositor

Michael Rosen

Article

Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Dcp1 (DCP1 Budding Yeast)

Tags

EGFP, TEV, 6xHis and MBP, TEV

Expression

Bacterial

Promoter

tac

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Article

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag7-SnoopLigase

Plasmid

#105627

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

HaloTag7-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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