30,753 results

1. pFW6000

   Plasmid

   #224215

   Purpose

   Bacteroides genomic editing tool; aTc inducible-Fncpf1

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18T-mini-Tn7T-hph-Ptac

   Plasmid

   #218364

   Purpose

   Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD_dGFPmut3-AAV

   Plasmid

   #187379

   Purpose

   arabinose-inducible expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

   Depositor

   Roi Avraham

   Insert

   dGFPmut3_AAV
   

   Tags

   AAV

   Expression

   Bacterial

   Mutation

   C-terminal AAV tail

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSET iGluSnFR3 v82

   Plasmid

   #175187

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 82 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v82
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

   Plasmid

   #135456

   Purpose

   Bacterial expression of uTEV2Δ protease

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
   

   Tags

   MBP, His6

   Expression

   Bacterial

   Mutation

   S219V mutation improves stability, T30A/S153N mut…

   Promoter

   tac promoter

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a 6xHis PreScission SARS-CoV-2 nsp13

   Plasmid

   #159390

   Purpose

   Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.

   Depositor

   Tarun Kapoor

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Insert

   N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIRT5-pET15b

   Plasmid

   #81030

   Purpose

   Bacterial expression of Human WT SIRT5 protein

   Depositor

   David Lombard

   Article

   Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

   Insert

   Sirt5 (SIRT5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pL0M-S-mTurquoise2-EC18152

   Plasmid

   #137074

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn7-155

    Plasmid

    #118569

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TH

    Plasmid

    #39079

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TH (TH Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pVP141

    Plasmid

    #222079

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP and sacB
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Sap-SH2

    Plasmid

    #46477

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sap (SH2D1A Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 4-128

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTBX-1

    Plasmid

    #26067

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSV2B

    Plasmid

    #118228

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-Ub4

    Plasmid

    #171421

    Purpose

    For the purifcation of tetra linear ubiquitin

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    Ubiquitin
    

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCpf1b

    Plasmid

    #122187

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAC-tag-C

    Plasmid

    #108077

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHTganA-Cpf1

    Plasmid

    #158647

    Purpose

    Constitutive transcription of FnCpf1 and ganA crRNA

    Depositor

    Wenjing Cui

    Insert

    Cpf1, ganA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Expression

    Bacterial

    Promoter

    P43

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AASDHPPT

    Plasmid

    #39192

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AASDHPPT (AASDHPPT Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only