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pET28.His.3C.86b.Tau2N4R

Plasmid

#226399

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet-MerTK-PTPN1

Plasmid

#197988

Purpose

Expression of MerTK Kinase Domain (wild-type) in E. coli

Depositor

Byung Il Lee

Article

Bae et al Exp Mol Med. 2022 Sep;54(9):1450-1460. d

Inserts

Tyrosine-Protein Kinase Mer (MERTK Human)
PTPN1 (PTPN1 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGI3EM31

Plasmid

#135491

Purpose

Agrobacterium T-DNA with Spun H2Bpr driving hph-mCerulean3

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr hph-mCerulean3

Tags

mCerulean3

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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human TF cDNA

Plasmid

#15455

Depositor

Nigel Mackman

Article

Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

Insert

Tissue factor (F3 Human)

Use

Luciferase

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDM2 (17-125)

Plasmid

#62063

Purpose

Expression of human MDM2 (17-125) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains residues 17-125

Promoter

tac

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pQE80L-IL2 C125S

Plasmid

#104348

Purpose

Expresses human IL-2 (C125S)

Depositor

Alexander McLellan

Insert

Human IL-2 (C125S) (IL2 Human)

Tags

MRGSHHHHHGS-tag

Expression

Bacterial

Mutation

C125S mutation (TGT -> TCC)

Promoter

T5

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-mCherry

Plasmid

#136615

Purpose

Dox-inducible lentivral vector expressing mCherry red fluorescent protein

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

mCherry

Use

Lentiviral

Expression

Bacterial

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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HOXA9-pRSET

Plasmid

#20979

Depositor

Corey Largman

Insert

HOXA9 (HOXA9 Human)

Tags

His and Xpress

Expression

Bacterial

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

GFP

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSR83

Plasmid

#90211

Purpose

Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

OmpR (ompR E. coli)
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-sfGFP

Plasmid

#163757

Purpose

Expresses Streptomyces codon-optimised sfGFP

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

SP44

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-std

Plasmid

#109417

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKCcas9dO

Plasmid

#62552

Purpose

High efficiency Streptomyces genome editing by CRISPR/Cas9 system

Depositor

Weihong Jiang

Article

Huang et al Acta Biochim Biophys Sin (Shanghai). 201

Inserts

Scocas9
sgRNA ()
act-orf4 homology arm

Use

CRISPR; Bacterial genome editing

Expression

Bacterial

Promoter

j23119 and ptipA

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMH1105

Plasmid

#131864

Purpose

Produces holo-PhyB-AviTag in E. coli

Depositor

Wilfried Weber

Article

Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

Inserts

PhyB(1-651)-AviTag-His6
HO1
PcyA

Expression

Bacterial

Promoter

T7 promoter-1 and T7 promoter-2

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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