30,753 results

1. pSB1A3_GFP_dT19

   Plasmid

   #89661

   Purpose

   expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

   Depositor

   Hendrik Dietz

   Article

   Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

   Insert

   GFP_dT19
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SHP-1(NC)-SH2

   Plasmid

   #46496

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-1(NC) (PTPN6 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-221

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. BSA1_His

   Plasmid

   #202766

   Purpose

   bovine serum albumin design BSA1, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   BSA1
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-Bcatfl

   Plasmid

   #24193

   Depositor

   David Rimm

   Insert

   beta-catenin (CTNNB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pGTVL2-CRBN-TBD

   Plasmid

   #196369

   Purpose

   protein expression of the GST-tagged Cereblon Thalidomide binding domain

   Depositor

   Stefan Knapp

   Insert

   CRBN (CRBN Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   contains only T319-T425

   Promoter

   T7

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30a(+)-PE2

   Plasmid

   #180015

   Purpose

   Expressing PE2 protein in E.coli for protein purification

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Lac operator-controlled T7 promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-6h-GST-TEVp

   Plasmid

   #172887

   Purpose

   Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Insert

   TEV protease
   

   Tags

   GST solubility tag and HHHHHH

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

   Plasmid

   #190182

   Purpose

   Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   iRFP
   hGeminin(1/110)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AK3L1

   Plasmid

   #39135

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AK3L1 (AK3 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pADsacA

    Plasmid

    #158649

    Purpose

    Constitutive transcription of sacA crRNA and GFP

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Expression

    Bacterial

    Promoter

    Pveg

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSU-araC-Cas9

    Plasmid

    #120423

    Purpose

    Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

    Depositor

    Sheng Yang

    Article

    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSET-dCas9

    Plasmid

    #110183

    Purpose

    Expression of the dCas9 gene in Streptomyces

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Insert

    dCas9
    

    Use

    CRISPR; I

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    ermE*p

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid

    #101215

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A and R1060A

    Promoter

    T7

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMV306hsp+FFluc

    Plasmid

    #26156

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    Firefly luciferase
    

    Expression

    Bacterial

    Mutation

    Firefly luciferase gene codon optimised for mycob…

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGR596

    Plasmid

    #213787

    Purpose

    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    See manuscript

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMV762-Peredox-mCherry

    Plasmid

    #90217

    Purpose

    Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

    Depositor

    Ashwani Kumar

    Article

    Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

    Insert

    Codon Optimized Peredox-mCherry
    

    Use

    Shuttle vector

    Expression

    Bacterial

    Promoter

    groEL

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAF1

    Plasmid

    #39117

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TAF1 (TAF1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pJH181.3

    Plasmid

    #135076

    Purpose

    Coryne. glutamicum HA production plasmid

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

    Insert

    hasA-hasC
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK1008 (pSL7)

    Plasmid

    #191003

    Purpose

    Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

    Depositor

    Jeffrey Barrick

    Article

    Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

    Insert

    red chromoprotein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    CP25

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hMSH2 Clone E3 (full-length)

    Plasmid

    #16453

    Depositor

    Bert Vogelstein

    Article

    Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

    Insert

    MSH2 (MSH2 Human)
    

    Expression

    Bacterial

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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