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31,059 results

Showing: 7761 - 7800 of 31059 results

  4. PP-GSK3

    Plasmid
    #25133
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd4_240:C29838
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. pCarCBADE_ProB(I69E)A

    Plasmid
    #117756
    Purpose
    ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
    Depositor
    Greg Bokinsky
    Article
    Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d
    Insert
    Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
    Expression
    Bacterial
    Promoter
    PLac(UV5)
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    117756-plasmid-map-sequence-id-230175

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  8. NUDT1

    Plasmid
    #74660
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT1 (NUDT1 Human)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  11. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  17. EYFP-Talin1-FERM

    Plasmid
    #110565
    Purpose
    bacterial expresion of EYFP-tagged Talin 1
    Depositor
    Tina Izard
    Insert
    Talin 1 (Tln1 Mouse)
    Tags
    (His)6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    110565-plasmid-map-sequence-id-213906

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  21. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

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  37. pJMP8930

    Plasmid
    #220905
    Purpose
    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220905-plasmid-map-sequence-id-436985

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Showing: 7761 - 7800 of 31059 results