32,811 results

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pBbA5c-MevT-MBI (JBEI-3085)

Plasmid

#87950

Purpose

the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

MevT-MBI

Expression

Bacterial

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAX1

Plasmid

#117397

Purpose

allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLG032 (ppl)

Plasmid

#157910

Purpose

Expresses the ppl defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ppl (PHP-ATPase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b-ALFA-TwinStrep

Plasmid

#240252

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Article

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, ALFA, Thrombin, and Twin-Strep

Expression

Bacterial

Promoter

T7

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pJJGL001

Plasmid

#180605

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

DpbCasX

Use

CRISPR

Tags

10x His and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK5-1/1-IS1/2

Plasmid

#91303

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS1/2 (DOCK5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptac

Plasmid

#218364

Purpose

Empty backbone for Tn7-site insertion with lacIq-Ptac expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1009 (pSL9)

Plasmid

#191004

Purpose

Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

Plasmid

#110556

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

anit-RD114 light chain

Tags

mKappa

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pET28CLY3

Plasmid

#21762

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 3 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
GST-AR-1-660

Plasmid

#104196

Purpose

Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

Insert

human androgen receptor aa 1-660 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q331K

Plasmid

#98673

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

Q331K

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAB076i7

Plasmid

#79201

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBBR4pemIK

Plasmid

#126928

Purpose

Broad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selection

Depositor

Lindsey Burbank

Article

Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4B-pCMV7.1

Plasmid

#155023

Purpose

N-terminal 3xFLAG-tagged CUL4B in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4B (CUL4B Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-GBFusion1

Plasmid

#133438

Purpose

Streptococcus GB1 domain in vector for bacterial expression.

Depositor

Gerhard Wagner

Article

Zhou et al J Biomol NMR. 2001 May . 20(1):11-4.

Insert

GB1 domain

Expression

Bacterial

Mutation

This fragment encodes only aa 1 to 56.

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-166

Plasmid

#118578

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pccGFPG83I

Plasmid

#73650

Purpose

Fluorescent reporter for transmembrane protein dimerization negative control.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A- G83I

Expression

Bacterial

Mutation

glycine 83 to isoleucine

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAB107a

Plasmid

#79218

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pgex-cIAP1

Plasmid

#8328

Depositor

Jon Ashwell

Article

Li et al Nature 2002 Mar 21;416(6878):345-7.

Insert

cIAP1 (BIRC2 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pBFC1207

Plasmid

#186694

Purpose

VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_2xBsaI_NT (Guide Stuffer) crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq

Expression

Bacterial

Promoter

PLac

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEC-K-CBP_CjeCas9

Plasmid

#83043

Purpose

Expresses Campylobacter jejuni Cas9 (Cje Cas9).

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CjeCas9

Expression

Bacterial

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only
pDA303

Plasmid

#195199

Purpose

Overexpressing 6His-TetR in E. coli.

Depositor

Rahul Sarpeshkar

Article

Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

Insert

TetR

Tags

6xHis

Expression

Bacterial

Promoter

T5 promoter

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCK005.6

Plasmid

#153025

Purpose

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

Use

CRISPR

Expression

Bacterial

Mutation

SoxS has R93A and S101A mutations

Available Since

Aug. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAE_14-3-3

Plasmid

#109942

Purpose

Protein expression and purification of YWHAE_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAE_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

Plasmid

#169076

Purpose

Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Inserts

ATG5 (ATG5 Human)
ATG7 (ATG7 Human)
ATG10 (ATG10 Human)
ATG12 (ATG12 Human)
ATG16 (ATG16L1 Human)

Tags

GGlinker, TEV cleavage site, StrepII and HIS10 Ta…

Expression

Bacterial and Insect

Promoter

Polyhedrin promoter

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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32,811 results