Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Bacterial Expression Clear filter

Applied Filters

Bacterial Expression Clear filter

32,811 results

Showing: 6241 - 6270 of 32811 results

  2. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

    Enlarge

  5. MARS_pNIC-CTB10H

    Plasmid
    #153053
    Purpose
    Expression construct for producing Avi tagged Methionyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    MARS (MARS1 Human)
    Tags
    TEV-Avi-His10
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153053-plasmid-map-sequence-id-304471

    Enlarge

  6. GST-PR-LBD

    Plasmid
    #89117
    Purpose
    bacterial expression of human progesterone receptor ligand binding domain amino acid residues 637-933 with GST tag in pGEX-3X-1
    Depositor
    Elizabeth Wilson
    Insert
    human progesterone receptor ligand binding domain (PGR Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    contains Ligand Binding Domain (637-933)
    Promoter
    tac
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89117-plasmid-map-sequence-id-175728

    Enlarge

  9. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

    Enlarge

  13. XXBLM (XXBLMA-c075)

    Plasmid
    #98223
    Purpose
    Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag
    Depositor
    Nicola Burgess-Brown
    Insert
    XXBLMA (BLM Human)
    Tags
    His-6
    Expression
    Bacterial
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98223-plasmid-map-sequence-id-193299

    Enlarge

  15. pGEM4Z-OMA1

    Plasmid
    #61718
    Purpose
    human Oma1 protease in the E.coli/in vitro translation vector
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Expression
    Bacterial
    Available Since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. p0857 pBR322 SV40

    Plasmid
    #10877
    Depositor
    Peter Howley
    Insert
    SV40
    Expression
    Bacterial
    Available Since
    Oct. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  20. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

    Enlarge

  24. USP15

    Plasmid
    #28282
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15 (USP15 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Contains dusp and Ubl domains (residues 6-223).
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. pJE53

    Plasmid
    #194153
    Purpose
    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.
    Depositor
    Edward Geisinger
    Article
    Bai et al J Bacteriol. 2021 May 20;203(12):e005652
    Insert
    sgRNA with mrfp control guide sequence
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    194153-plasmid-map-sequence-id-382435

    Enlarge

Showing: 6241 - 6270 of 32811 results