32,161 results
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Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQIq_MRGS_HIS8_(27F3)_FLAG
Plasmid#199615PurposeEncodes DARPin-FLAG 27F3 for bacterial expression and column purificationDepositorInsertDARPin-FLAG 27F3
Tags8xHis and FLAGExpressionBacterialPromoterT5Available SinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-ALFA-TwinStrep
Plasmid#240248PurposeArabinose-inducible plasmid with ALFA and Twin-Strep tagsDepositorTypeEmpty backboneTags10x HIS, ALFA, Thrombin, and Twin-StrepExpressionBacterialPromoteraraBADAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMVRha
Plasmid#229553PurposeBroad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYSD063
Plasmid#212916PurposeEncodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertmRuby2
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
Tags6xHis and Twin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-SENP2
Plasmid#196355Purposeprotein expression of the His-tagged catalytic domainDepositorInsertSENP2 catalytic domain
TagsHis6-TEVExpressionBacterialMutationcontains only D364-L589PromoterT7Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT-2
Plasmid#22833DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
TRBV20-1
Plasmid#123454Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Rin1-SH2
Plasmid#46474DepositorInsertRin1 (RIN1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 54-204PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
PLlacO-1-mfLon
Plasmid#45152DepositorInsertLon
UseSynthetic BiologyTagsssRA degradation (LVA)ExpressionBacterialPromoterPLlacO-1Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMis1_1B
Plasmid#192079PurposeA pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent inductionDepositorInsertsKanR
rhaR
rhaS
pBBR1 rep
ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL723
Plasmid#140393PurposeExpresses HucR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-HucR-T
Tags6XHisExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
NRAS
Plasmid#55654Purposeexpression of mouse NRASDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64TS (DM#117)
Plasmid#15034DepositorInsertbeta-globin 5' and 3' UTR
ExpressionBacterialAvailable SinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pCadBA-mCherry
Plasmid#192865PurposepMUT2 based vector for sensing pH in E. coli Nissle 1917DepositorInsertpH sensitive promoter
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
TagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Sap-SH2
Plasmid#46477DepositorInsertSap (SH2D1A Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 4-128PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-AmtR
Plasmid#49355DepositorInsertAmtR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRM053 His-TEV-Cas9 triNLS 0C
Plasmid#196244PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
His 3C MKK3 1-347
Plasmid#87878PurposeE. coli expression of MKK3 aa 1-347DepositorInsertMKK3 (MAP2K3 Human)
Tags3C protease site and 6xHisExpressionBacterialMutationaa 1-347PromoterT7Available SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-PUM1-HD
Plasmid#17543DepositorAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
ExpressionBacterialPromoterLambda Pr and Lambda PrmAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
EIF5A
Plasmid#25260PurposeBacterial expression for structure determination; may not be full ORFDepositorInserteIF5A:S15-A151 (EIF5A Human)
TagsHisExpressionBacterialMutationContains amino acids S15-A151Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
6his-WT gusA-pET28a+
Plasmid#61156PurposeN -terminal His tagged wild-type GUS expression vectorDepositorInsertgusA
Tags6x HisExpressionBacterialPromoterT7Available SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only