33,449 results

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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Publication

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pG1AK

Plasmid

#71736

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSLC-237

Plasmid

#73191

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

Depositor

Swaine Chen

Publication

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

ParaB-relE

Expression

Bacterial

Promoter

ParaB

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-USP11

Plasmid

#78849

Purpose

To purify GST-tagged recombinant USP11 protein

Depositor

Jaewhan Song

Publication

Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

Insert

USP11 (USP11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-RIPK3

Plasmid

#78827

Purpose

To purify GST-tagged recombinant RIPK3 protein

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Publication

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Expression

Bacterial

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Publication

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pTrcECT

Plasmid

#75477

Purpose

Inducible expression of ectABC in E. coli

Depositor

Xixian Xie

Publication

Ning et al Metab Eng. 2016 Mar 9;36:10-18. doi: 10.

Insert

ectABC

Expression

Bacterial

Promoter

trc

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO tTR-KRAB-rtTA3-Luc

Plasmid

#68449

Purpose

TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

tTR-KRAB-2A-rtTA3-2A-Luciferase

Use

Luciferase; Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-MAP

Plasmid

#66999

Purpose

Expresses malE-MAP in E. coli

Depositor

Ashutosh Chilkoti

Publication

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-MAP

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRL692

Plasmid

#70284

Purpose

Tn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942

Depositor

Peter Wolk

Publication

Koksharova et al J Bacteriol. 2002 Oct;184(19):5524-8.

Insert

Transposon Tn5-692

Expression

Bacterial

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Publication

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Publication

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Publication

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAT593

Plasmid

#68909

Purpose

NanoLuc expressed constiutively from PBT1311 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Publication

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pB3.P11

Plasmid

#69577

Purpose

OT-I TCR beta chain cDNA in pCRScript

Depositor

Francis R. Carbone

Publication

Hogquist et al Cell. 1994 Jan 14;76(1):17-27.

Insert

TCR beta cDNA clone B3 (Tcrb Mouse)

Expression

Bacterial

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBSKrnaSeph

Plasmid

#67506

Purpose

Express the tRNALys scaffold with the sephadex aptamer tag

Depositor

Luc Ponchon

Publication

Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

Insert

modified tRNALys with sephadex aptamer

Expression

Bacterial

Promoter

lpp

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
Orange Nano-lantern/pRSETB

Plasmid

#67659

Purpose

orange luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Orange Nano-lantern

Tags

6xHis

Expression

Bacterial

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pLJSRSF3

Plasmid

#64692

Purpose

His-GST-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Publication

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, GST, HisTag, NpuDnaE-N102, and Smt3

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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