34,213 results
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Plasmid#88901PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + Red-shifted firefly luciferase
ExpressionBacterialAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
ExpressionBacterialPromoterLacUV5 and pBADAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cIopt-J23106-geneIII
Plasmid#80852PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cIopt
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJPC12-ΔM13-mCherry-PR/PRM-GFP
Plasmid#80859PurposeReporter plasmid (mCherry and GFP)DepositorInsertsmCherry
GFP
ExpressionBacterialPromoterLambda Pr and Lambda PrmAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
DYR1A_HUMAN_D0
Plasmid#79690PurposeThis plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30-2-GPD1
Plasmid#83911Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
Tags6x His tagsExpressionBacterialPromoterT7Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB076i5
Plasmid#79199PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI-J23106-geneIII
Plasmid#80843PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cI
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-SdeA
Plasmid#80837PurposeBacterial expression of SdeADepositorInsertSdeA
Tags6xHisExpressionBacterialPromoterT5Available sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-LOVSD
Plasmid#81033PurposeMammalian expression of the super-dark state of LOV2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOVSD
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450A, L514K, G528A, L531E, N538EAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pG1AK
Plasmid#71736PurposeModular shuttle vector for Geobacillus and E. coliDepositorPublicationTypeEmpty backboneUseSynthetic Biology; Shuttle vectorExpressionBacterialAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLC-237
Plasmid#73191PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.DepositorInsertParaB-relE
ExpressionBacterialPromoterParaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-USP11
Plasmid#78849PurposeTo purify GST-tagged recombinant USP11 proteinDepositorAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-RIPK3
Plasmid#78827PurposeTo purify GST-tagged recombinant RIPK3 proteinDepositorAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAN3945
Plasmid#74699PurposeCircuit plasmid: 0x41DepositorInsertCircuit 0x41
ExpressionBacterialAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAL-rfp_T
Plasmid#74075PurposepAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecRDepositorPublicationInsertsgRNA rfp (T)
UseSynthetic BiologyExpressionBacterialPromoterptacAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only