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31,059 results

Showing: 9121 - 9160 of 31059 results

  7. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  10. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  17. Sperm whale Mb 64Y68F in pVP80K

    Plasmid
    #86956
    Purpose
    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).
    Depositor
    John Olson
    Insert
    Mb 64Y68F
    Expression
    Bacterial
    Mutation
    64Y68F
    Promoter
    T5
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86956-plasmid-map-sequence-id-176792

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  18. pCJ207

    Plasmid
    #162680
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162680-plasmid-map-sequence-id-321455

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  24. 6ASB (FBXL19)

    Plasmid
    #119954
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    FBXL19 (FBXL19 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 31-153
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119954-plasmid-map-sequence-id-232625

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  33. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Tags
    PAmCherry
    Expression
    Bacterial
    Promoter
    Ppen
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

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  35. MTST6

    Plasmid
    #86603
    Purpose
    Custom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.
    Depositor
    Mary Ann Moran
    Article
    Satinsky et al Methods Enzymol. 2013;531:237-50. doi: 1
    Insert
    MTST6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    86603-plasmid-map-sequence-id-193515

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  38. pTXB1-hMeCP2

    Plasmid
    #48091
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-WT
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pET14b_tev_PDZ1

    Plasmid
    #59739
    Purpose
    expresses PDZ1 domain of human ZO-1 in bacteria
    Depositor
    Alan Fanning
    Insert
    zonula occludens-1 (TJP1 Human)
    Tags
    HIS tag and thrombin clvg
    Expression
    Bacterial
    Available Since
    Sept. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 9121 - 9160 of 31059 results