31,059 results
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pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)
Plasmid#218513Purposereplicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferaseDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of the Plac_mrfp cassettePromoterPgyrA_ermBL-ermB'Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADA
Plasmid#39020PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQdC12a
Plasmid#191356PurposeClostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expressionDepositorInsertsPGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR
ExpressionBacterialPromoterPGus2A-TetO2/1 and miniPthlAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG8Ha-EWS-FLI1_Type2
Plasmid#188042PurposeExpresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.DepositorAvailable SinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSKB3.MPN348
Plasmid#11437DepositorInsertmethenyltetrahydrofolate synthetase
Tags6x His and TEV cleavage siteExpressionBacterialAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pKP864
Plasmid#167658PurposeExpress colicinB-His6DepositorInsertcba-his6
ExpressionBacterialMutationcba-his6Available SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)
Plasmid#48285DepositorTypeEmpty backboneTagsHis6ExpressionBacterialAvailable SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET_MBP-RGG-GFP-RGG
Plasmid#124949PurposeExpression of MBP-RGG-GFP-RGGDepositorInsertMBP-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
TagsEGFP, His, and MBPExpressionBacterialAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFDH
Plasmid#131706PurposeExpress constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coliDepositorInsertformate dehydrogenase
Tagshis-tagExpressionBacterialAvailable SinceNov. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD-NStrepHis
Plasmid#45936Purposeuse to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tagDepositorTypeEmpty backboneTags6xHis and Strep IIExpressionBacterialPromoterTrc (lactose/IPTG inducible)Available SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL
Plasmid#187712PurposeExpress GST-tagged NEDD4L FL in bacterial cellsDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV109
Plasmid#215339PurposeExpresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPCinAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
ExpressionBacterialPromoternoneAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM10686
Plasmid#213995PurposePrpsM driven expression of mScarlet-IDepositorInsertmScarlet-I
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
eNS2B47NS3 L30SF31S
Plasmid#155319PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only
