34,218 results

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pmVirD2

Plasmid

#90314

Purpose

mVirD2 expression plasmid

Depositor

Bjorn Hogberg

Publication

Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

Insert

A. tumefaciens VirD2 from aa 1 to 204

Tags

His6x tag

Expression

Bacterial

Promoter

T7/T5 IPTG inducible

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSETb-Ferritin-Dronpa

Plasmid

#101194

Purpose

Expresses a Ferritin-Dronpa fusion construct in E. coli

Depositor

Peter Dedecker

Publication

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

Ferritin-Dronpa

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Publication

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Publication

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Bacterial

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAV126

Plasmid

#63192

Purpose

pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Publication

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_12E

Plasmid

#98652

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Publication

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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K84M kinase-dead Nuak1-3xflag

Plasmid

#97219

Purpose

Plasmid expressing kinase-dead mutant Nuak1

Depositor

Huda Zoghbi

Publication

Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nuak1 (NUAK1 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Kinase-dead K84M

Promoter

T7

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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371-GFP

Plasmid

#97030

Purpose

His6-GFP-tev-yORF

Depositor

Chris Jeans

Type

Empty backbone

Tags

GFP fusion, His6 tag, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ppr_Cas13a_WT

Plasmid

#91872

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

PprCas13a (PprC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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BPTF PHD and Bromodomain

Plasmid

#92101

Purpose

Expresses GST fusion PHD and Bromodomain from BPTF protein

Depositor

Albert Jeltsch

Publication

Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

Insert

PHD and Bromodomain (BPTF Human)

Tags

GST Tag

Expression

Bacterial

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Erik Rodriguez, Roger Tsien

Publication

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl

Plasmid

#87688

Purpose

Express PhoCl in E. coli

Depositor

Robert Campbell

Publication

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl

Tags

6xHis

Expression

Bacterial

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAB094a

Plasmid

#79241

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PBAD rpoZ-HA4; PC araC

Expression

Bacterial

Promoter

PBAD; PC

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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WT C-His Ana GvpC in pET28a

Plasmid

#85732

Purpose

Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Publication

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added an extra glycine after the methionine at th…

Promoter

T7

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Publication

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ubiquitin K63C

Plasmid

#86589

Purpose

Bacterial expression of Ubiquitin K63C for cross linking studies

Depositor

Sharon Rozovsky

Publication

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

Ubiquitin (UBC Human)

Tags

His6-MBP

Expression

Bacterial

Mutation

TEV site; K63C

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Publication

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Publication

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBiex-1 AC30_eGFP

Plasmid

#82715

Purpose

Expresses a GFP-tagged recombinant adenylyl cyclase.

Depositor

Sivaraj Sivaramakrishnan

Publication

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Publication

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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