30,536 results

1. pET-alpha-synuclein-BiFC

   Plasmid

   #87855

   Purpose

   pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   alpha-synuclein-VC155
   alpha-synuclein-VN154
   

   Use

   Tags

   C-terminal fragment of mVenus BiFC protein and N-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-DEST

   Plasmid

   #119749

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRK669

   Plasmid

   #71313

   Purpose

   Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-MTF-His

   Plasmid

   #124133

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionyl-tRNA formyltransferase (fmt Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE30-His-ThrRS

   Plasmid

   #124119

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Threonyl-tRNA ligase (thrS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-NTOM20-mVenus-Zdk2

   Plasmid

   #81011

   Purpose

   Expression of Zdk2 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk2
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. BRD4

   Plasmid

   #38943

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK503

   Plasmid

   #110616

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKD4_mCherry

   Plasmid

   #187386

   Purpose

   To chromosomally tag genes of interest with mCherry. FRT-mCherry-kan-FRT

   Depositor

   Roi Avraham

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mOrange2-pBAD

    Plasmid

    #54531

    Purpose

    Localization: Protein Expression Vector , Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    6xHis and mOrange2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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