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pTAT-HA

Plasmid

#35612

Depositor

Steven Dowdy

Publication

Nagahara et al Nat Med. 1998 Dec;4(12):1449-52.

Type

Empty backbone

Tags

6xHIS, HA, and TAT

Expression

Bacterial

Promoter

T7

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p5068 pGEX-6P-1 Brd4 full-length

Plasmid

#14447

Depositor

Peter Howley

Publication

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

GST

Expression

Bacterial

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GST-MNase

Plasmid

#136291

Purpose

Expresses Micrococcal nuclease

Depositor

Kavitha Sarma

Publication

Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

Insert

MNase

Expression

Bacterial

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Robert Singer, Evelina Tutucci

Publication

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-BirA*-dCas9-eGFP

Plasmid

#84779

Purpose

Expresses BirA*-dCas9-eGFP in mammalian cells.

Depositor

Heinrich Leonhardt

Publication

Schmidtmann et al Nucleus. 2016 Sep 27:0.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA*-dCas9-eGFP

Use

CRISPR

Tags

cMyc

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

July 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFC334

Plasmid

#87846

Purpose

AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
argB
yA specific sgRNA

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

A. nidulans gpdA promoter with Hammerhead ribozym…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Purpose

Expresses hM4D (Gi) neuronal inhibitor

Depositor

Bryan Roth

Publication

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hM4D (Gi)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pET MBP His6 LIC cloning vector (2Cc-T)

Plasmid

#37237

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET28-MHL

Plasmid

#26096

Purpose

SGC Empty backbone for bacterial expression

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Tags

6xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Aug. 20, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

21 Articles

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pB33eCPX

Plasmid

#23336

Depositor

Patrick Daugherty

Publication

Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

Insert

Enhanced Circularly Permuted Outer Membrane Protein X

Expression

Bacterial

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Publication

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-pAG-Hia5

Plasmid

#174373

Purpose

Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Publication

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein AG - hia5

Tags

6xHis and Protein A/G

Expression

Bacterial

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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SpyDock

Plasmid

#124618

Purpose

Expresses SpyDock to bind SpyTag non-covalently for affinity purification

Depositor

Mark Howarth

Publication

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyDock

Tags

His6

Expression

Bacterial

Mutation

E77A mutation prevents isopeptide bond formation,…

Promoter

T7

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15bpATnp

Plasmid

#121137

Purpose

This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

Depositor

Keji Zhao

Publication

Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

Insert

Protein A-Tnp

Tags

his

Expression

Bacterial

Promoter

T7

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Publication

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSmartBAC-S

Plasmid

#107268

Purpose

Broad host range bacterial artificial chromosome vector

Depositor

Ron Godiska, Mark Liles

Publication

Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

Type

Empty backbone

Expression

Bacterial

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNCS-Antares

Plasmid

#74279

Purpose

Constitutive expression of Antares in bacteria

Depositor

Michael Lin

Publication

Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

Antares

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET-28a-hPKM2

Plasmid

#44242

Depositor

Lewis Cantley, Matthew Vander Heiden

Publication

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRsetB-His7tag-Peredox-mCherry

Plasmid

#32382

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

His7-Peredox-mCherry

Tags

His7tag

Expression

Bacterial

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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