30,249 results

1. pRPN265NB-LaG16-MNase

   Plasmid

   #170978

   Purpose

   Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

   Depositor

   Marc Timmers

   Article

   Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

   Insert

   Glutathione-S-Transferase/LaG16/MNase
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Jason Peters, Carol Gross, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD3

   Plasmid

   #45604

   Depositor

   Barry L. Wanner

   Article

   Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

   Insert

   FRT-cat-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   R6K

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-pBAD

   Plasmid

   #54762

   Purpose

   Localization: Protein Expression Vector , Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. plRL19

   Plasmid

   #163634

   Purpose

   L5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Insert

   L5 Integrase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Mycobacterial Optimized Promoter (MOP) and Tet-On

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDB302

   Plasmid

   #113673

   Purpose

   expresses Cth SUMO protease in Escherichia coli

   Depositor

   Christopher Bahl

   Article

   Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

   Insert

   Cth SUMO protease
   

   Use

   Tags

   10-His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-934

   Promoter

   T7

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrc-trGPPS(CO)-LS

   Plasmid

   #50603

   Purpose

   pTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthase

   Depositor

   Jay Keasling

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Inserts

   Limonene Synthase
   ApR
   LacIq
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   truncated codon optimized geranylpyrophosphate sy…

   Promoter

   Trc

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPSU150

   Plasmid

   #173836

   Purpose

   Expresses 150 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABMBPpepN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-RT003-GPM

   Plasmid

   #163383

   Purpose

   To express sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   GFP-PDGFRtm-2A-mCherry (GPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAP03-acc(3)IV

    Plasmid

    #69862

    Purpose

    pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

    Depositor

    Bradley Moore

    Article

    Tang et al ACS Chem Biol. 2015 Oct 21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-p47PX(aa2-132)

    Plasmid

    #19007

    Depositor

    Michael Yaffe

    Article

    Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

    Insert

    p47phox (NCF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa2-132 (PX domains)

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCold TDP43 WT

    Plasmid

    #27451

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa, GST, and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Opto-CRAC version 1

    Plasmid

    #101245

    Purpose

    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

    Depositor

    Yubin Zhou

    Article

    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

    Insert

    LOV2 and STIM1 cytosolic fragment
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 Sumo TEV LIC cloning vector (1S)

    Plasmid

    #29659

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Jason Peters, Carol Gross, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAK1

    Plasmid

    #73244

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AAK1 (AAK1 Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 47-307

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. T7 promoter + terminators reporter plasmid

    Plasmid

    #156456

    Purpose

    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

    Inserts

    NeoR/KanR
    TcR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ATG start codon mutated to ACG

    Promoter

    Em7 and tet

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. OPEN Beta2-microglobulin

    Plasmid

    #215657

    Purpose

    E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN Beta2-microglobulin (B2M Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 31 to Cysteine

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-NLS-Cas9-6xHis

    Plasmid

    #62934

    Purpose

    Expression of NLS-Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    NLS-Cas9-6xHis
    

    Use

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCre-SacB-zeo

    Plasmid

    #107706

    Purpose

    Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Insert

    ble (Zeo resistance)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge