30,733 results

1. pKD239-1g-2

   Plasmid

   #90959

   Purpose

   Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Tetrathionate response regulator
   Superfolder GFP
   mCherry
   

   Expression

   Bacterial

   Promoter

   Bba_J23100, Bba_J23109, and PttrB185-269

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-NLyn-Venus-Zdk1

   Plasmid

   #81042

   Purpose

   mammalian expression of plasma-membrane anchored Zdk1

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk1
   

   Tags

   6x His tag, FLAG, NLyn, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6p-1-UFBP1

   Plasmid

   #204558

   Purpose

   Expresses GST-tagged UFBP1 in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFBP1 (DDRGK1 Human)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE2-TetOminiCMV-mCherry

   Plasmid

   #136615

   Purpose

   Dox-inducible lentivral vector expressing mCherry red fluorescent protein

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET FLIPPi-5u

   Plasmid

   #13554

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi S52A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   S52A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPSU10-30-50-100

   Plasmid

   #173838

   Purpose

   Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPAB
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUdO1k

   Plasmid

   #210234

   Purpose

   high-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS0806 His14-bdSUMO-AwaIscB

   Plasmid

   #176539

   Purpose

   Bacterial expression of His14-bdSUMO-AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   CRISPR

   Tags

   14xHis-bdSUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUdO2c

    Plasmid

    #210231

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mCherry-LOV2 V416I

    Plasmid

    #81045

    Purpose

    mammalian expression of LOV V416I

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416I
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416I

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)
    

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pgWIZ-SGCAgl

    Plasmid

    #131456

    Purpose

    Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    SGCA (SGCA Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial and Mammalian

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pTGV-Express-CAT SMR5916

    Plasmid

    #28039

    Depositor

    Susan Rosenberg

    Article

    Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSSCP

    Plasmid

    #183190

    Purpose

    mRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEV

    Depositor

    Hsiao-Chun Huang

    Article

    Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

    Inserts

    mRFP-PopZ
    DivIVA-GFP(tevR-50)
    SpmX-TEV(C)
    TEV(N)-SpmX
    

    Expression

    Bacterial

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSU313

    Plasmid

    #169695

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_UBA52_Ubiquitin

    Plasmid

    #110043

    Purpose

    Protein expression and purification of UBA52_Ubiquitin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    UBA52_Ubiquitin (UBA52 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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