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Bacterial Expression

29,776 results

Showing: 4841 - 4860 of 29776 results

  1. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Use
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    137046-plasmid-map-sequence-id-292071

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  6. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid
    #122850
    Purpose
    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli
    Depositor
    Martin Grininger
    Article
    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi
    Insert
    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122850-plasmid-map-sequence-id-238544

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  7. pGBW-m4046899

    Plasmid
    #145608
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145608-plasmid-map-sequence-id-292566

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  10. peSCBE3-NG-Hypa

    Plasmid
    #218165
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218165-plasmid-map-sequence-id-433201

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  11. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVvanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  12. 6CCR (GID4)

    Plasmid
    #119960
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    GID4 (C17orf39 Human)
    Use
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 116-300
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119960-plasmid-map-sequence-id-232678

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  14. pOPINE-3C-eGFP

    Plasmid
    #41125
    Depositor
    Ray Owens
    Insert
    GFP
    Use
    Tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41125-plasmid-map-sequence-id-54698

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  15. pYTK081

    Plasmid
    #65188
    Purpose
    Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Insert
    CEN6/ARS4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65188-plasmid-map-sequence-id-110241

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  16. PylRS-AS

    Plasmid
    #137908
    Purpose
    For incorporation of fluorophenylalanine derivatives
    Depositor
    Wenshe Liu
    Article
    Lee et al Chem Commun (Camb). 2016 Oct 18;52(85):1
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Containing N346A, C348S mutations
    Promoter
    pBAD
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    137908-plasmid-map-sequence-id-272329

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  17. RBD#3L

    Plasmid
    #193020
    Purpose
    hnRNP A1 RRM2 RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a
    Depositor
    Xue Gao
    Article
    Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038
    Insert
    Cas13a
    Use
    Tags
    hnRNP A1 RRM2
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    193020-plasmid-map-sequence-id-380237

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Showing: 4841 - 4860 of 29776 results