31,587 results
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Plasmid#177334Purposebacterial expression of active SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
TagsFactor Xa site-3xFlag-Myc-6xHisExpressionBacterialMutationsilent mutation C to T at position 10546 (elimina…PromoterT7Available SinceNov. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
esmGFP-C10-pVTCB052
Plasmid#231303PurposePlasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coliDepositorInsertesmGFP-C10
ExpressionBacterialAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAJM.847
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
ExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NXF1_TAP-C
Plasmid#110038PurposeProtein expression and purification of NXF1_TAP-CDepositorInsertNXF1_TAP-C (NXF1 Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LIC
Plasmid#62303PurposeE. coli expression vector with N-terminal Hexa-HIS and TEV cleavageDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132)
Plasmid#73742PurposeExpression of human RPN13 PRU domain in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationPRU domain only (residues 19-132)PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Mocr TEV LIC cloning vector (1O)
Plasmid#29658DepositorTypeEmpty backboneTags6xHis, Mocr, and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TadA*-T7RNAP
Plasmid#167980PurposeExpresses fusion protein TadA*-T7RNAP in E. coliDepositorInsertTadA*-T7RNAP
TagsT7 tag and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-eBFP2
Plasmid#102640PurposeIn vitro expression of eBFP2 from the T7 promoterDepositorInserteBFP2
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
NRASA
Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
TagsHisExpressionBacterialMutationContains amino acids M1-N172Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pET-28a(+)-T7-OmpA-MNaseB-6xHis
Plasmid#214809PurposeExpression of 6xhis-tagged micrococcal nuclease B (MNaseB)DepositorInsertOmpA-Micrococcal nuclease B
Tags6xhisExpressionBacterialPromoterT7 promoterAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NAD-Snifit
Plasmid#113658PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentrationDepositorInsertNAD-Snifit
ExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPM2213
Plasmid#167553PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12DepositorInsertrfb region of Shigella flexneri serotype 2a
ExpressionBacterialAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Omega 1
Plasmid#118046PurposeGoldenBraid2.0 Omega 1 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…ExpressionBacterial, Insect, and Mamm…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-P43
Plasmid#112775PurposeStrong promoter activity during exponential and stationary phaseDepositorArticleInsertP43 promoter
ExpressionBacterialAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -