30,296 results

1. pET28sumo-hRig-I DCARDS

   Plasmid

   #210828

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

   Insert

   hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCP20 PilB

   Plasmid

   #162845

   Purpose

   Expression of PilB with a C-terminal histag

   Depositor

   Katrina Forest

   Insert

   PilB
   

   Use

   Tags

   Histidine tag (H6x)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. tracrRNA(scr)-CJ8421_04975 mRNA

   Plasmid

   #199718

   Purpose

   Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   CJ8421_04975 mRNA and scrambled tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSU315

   Plasmid

   #169697

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   1xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-DDX43

   Plasmid

   #134574

   Purpose

   Express human DDX43 protein (WT, full-length) in E. coli

   Depositor

   Yuliang Wu

   Article

   Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

   Insert

   DDX43 (DDX43 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   full-length WT

   Promoter

   T7

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-PRMT1

   Plasmid

   #34699

   Depositor

   Steven Clarke

   Article

   Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

   Insert

   PRMT1 (Prmt1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET15b T7pCONS TIR-2 sfGFP

   Plasmid

   #154468

   Purpose

   pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-LgBiT-SpyCatcher

    Plasmid

    #223634

    Purpose

    Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6, LgBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET32a-hTGFa

    Plasmid

    #199236

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Transforming growth factor alpha, partial (TGFA Human)
    

    Use

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRG/III-hs-MBP

    Plasmid

    #121676

    Purpose

    Bacterial expression of MBP fusion proteins

    Depositor

    Reinhard Grisshammer, Richard Henderson

    Article

    Tucker et al Biochem J. 1996 Aug 1;317 ( Pt 3):891-9.

    Insert

    maltose binding protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET-WHaloCaMP1b-EGFP

    Plasmid

    #205305

    Purpose

    Expression of WHaloCaMP1b-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1b-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Human, Synthetic)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLG021 (AVAST type 5)

    Plasmid

    #157899

    Purpose

    Expresses the AVAST type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 5 (STAND ATPase with N-terminal SIR2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ESCRT-I

    Plasmid

    #17634

    Depositor

    James Hurley

    Article

    Kostelansky et al Cell. 2006 Apr 7. 125(1):113-26.

    Insert

    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101) (VPS28, STP22, SRN2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-T-rnVAPA-1-125

    Plasmid

    #13396

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pT7RNAP-Q737P

    Plasmid

    #175356

    Purpose

    Support plasmid for T7_ori plasmids

    Depositor

    Sven Panke

    Article

    Becker et al Nucleic Acids Res. 2021 Jul 13. pii: 632

    Insert

    T7RNAP-Q737P
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q737P

    Promoter

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge