30,804 results

1. IRES-creERT2-FNF

   Plasmid

   #188230

   Purpose

   EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette

   Depositor

   Mario Capecchi

   Insert

   IRES-creERT2-FNF
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDx_mScarlet-I3-NCwt-SYFP2

   Plasmid

   #189765

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3-NCwt-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AdML

   Plasmid

   #11242

   Depositor

   Robin Reed

   Article

   Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

   Insert

   AdML
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCoofy4

   Plasmid

   #43986

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbA8a-RFP

   Plasmid

   #35271

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   BAD

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQE60 MGH

   Plasmid

   #204244

   Purpose

   Bacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MGH
   

   Tags

   6X His and GFP

   Expression

   Bacterial

   Mutation

   fusion of MDH and GFP with His tag

   Promoter

   Lac

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSG86-J23151-LasR

   Plasmid

   #102456

   Purpose

   Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   LasR (lasR )
   

   Expression

   Bacterial

   Promoter

   J23151

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJMP2832

   Plasmid

   #160672

   Purpose

   Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   gmc6 (gfp) sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJMP2820

   Plasmid

   #160669

   Purpose

   Mobile CRISPRi sfGFP 'test', empty sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   empty sgRNA (BsaI)
   

   Expression

   Bacterial

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMRE142

    Plasmid

    #118494

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. UHRF1

    Plasmid

    #28163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1 (UHRF1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    tandem tudor domain

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. ILF2_DZF_pGEX

    Plasmid

    #135162

    Purpose

    Recombinant expression of ILF2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    ILF2 (ILF2 Human)
    

    Tags

    GST-SBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pREDKI

    Plasmid

    #51626

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Expression

    Bacterial

    Promoter

    pRha

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTriExEKAR2G2

    Plasmid

    #40175

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid

    #41733

    Purpose

    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    T7

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. APneg-VAMP1b

    Plasmid

    #168008

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. APneg-VAMP1c

    Plasmid

    #168009

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVEX-CC1

    Plasmid

    #74170

    Purpose

    protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Tags

    None

    Expression

    Bacterial

    Promoter

    Inducible Tac promoter

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pIB165

    Plasmid

    #90188

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Zap70(NC)-SH2

    Plasmid

    #46535

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Zap70(NC) (ZAP70 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-268

    Promoter

    Tac

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge