30,261 results

1. MYH11-gRNA1

   Plasmid

   #132587

   Purpose

   gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

   Depositor

   James Thomson

   Article

   Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

   Insert

   MYH11-gRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuperClomeleon

   Plasmid

   #203724

   Purpose

   An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

   Depositor

   George Augustine

   Article

   Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

   Insert

   Cerulean linked topaz fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Cerulean, modified Topaz Fluorescent Protein with…

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-His-Dre

   Plasmid

   #123130

   Purpose

   Bacterial expression plasmid for wild-type Dre with an N-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   Dre recombinase
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19b+H3

   Plasmid

   #157807

   Purpose

   Expression of cysteine-free human histone H3 (c111A)

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C111A

   Promoter

   T7

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. SHMT2

   Plasmid

   #25479

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A269T

   Promoter

   Available since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. SB_convex

   Plasmid

   #110104

   Purpose

   pBXPHM3 with scaffold Sybody of the convex library

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Insert

   scaffold Sybody of the convex library
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AF1521-c003

   Plasmid

   #173111

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
   

   Use

   Tags

   Nanoluc

   Expression

   Bacterial

   Mutation

   KH-M1-L192

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWP78-PVCpnf_pvc13-ALFA

   Plasmid

   #198301

   Purpose

   Produces PVCs retargeted with ALFA tag

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-ALFA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHimar6

   Plasmid

   #137081

   Purpose

   bacterial Himar1 mini-transposon donor plasmid

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar1 mini-transposon with chloramphenicol resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGCP123-GFP_g1

    Plasmid

    #153518

    Purpose

    sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE102

    Plasmid

    #59397

    Purpose

    MethyloBrick expression vector, mxaF promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PmxaF

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-Caspase-3(C163S)

    Plasmid

    #90088

    Purpose

    Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C163S

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pECJ3

    Plasmid

    #75465

    Purpose

    Toggle switch plasmid containing lacI-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    lacI-pdt3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQF

    Plasmid

    #48095

    Purpose

    Cumate-inducible expression of 3xFLAG-tagged fusion protein

    Depositor

    Julia Vorholt

    Article

    Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    PQ5

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. TurboRFP-pBAD

    Plasmid

    #54858

    Purpose

    Localization: Protein Expression Vector , Excitation: 553, Emission: 574

    Depositor

    Dmitriy Chudakov, Dorus Gadella, Michael Davidson

    Article

    Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

    Type

    Empty backbone

    Use

    Tags

    6xHis and TurboRFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    superfolder GFP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG022 (dsr1)

    Plasmid

    #157900

    Purpose

    Expresses the dsr1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    dsr1 (SIR2-DUF4020)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETB-roGFP1-R12

    Plasmid

    #83311

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

    Insert

    roGFP1-R12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

    Promoter

    T7

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

19. D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

    Plasmid

    #81185

    Purpose

    Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Inserts

    Cas5c
    Cas8c
    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMMLV-EBFP-P2A-Puro

    Plasmid

    #160227

    Purpose

    Expresses EBFP in mammalian cells

    Depositor

    Jason Sheltzer

    Article

    Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

    Insert

    EBFP-P2A-Puro
    

    Use

    Retroviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge