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Plasmid#104333PurposeExpresses taz from a LacI regulated promoter under inducible IPTG control.DepositorInsertTaz
UseTagsExpressionBacterialMutationPromoterPtacIAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
UseTagsHis-thrombinExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-3C-irisin
Plasmid#122612PurposeExpresses in E. coli: human irisin with Nt histag and 3C protease site for cleavageDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Rat, Mouse, Bovine, Human)
UseTagsNt: histag-3C protease siteExpressionBacterialMutationPromoterT7 polymeraseAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Grb2 1-154 (XSB382)
Plasmid#78510PurposeConstruct for expressing Grb2 lacking the C-terminal SH3 domain in E. ColiDepositorInsertGrb2 1-154 (GRB2 Human)
UseTagsGST followed by a prescission protease claevage s…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
1457_pDEST_miniTol2_R4-R2_Cryst_mCherry
Plasmid#171796PurposepDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-jAspSnFR3
Plasmid#203458PurposejAspSnFR3 in T7 bacterial expression vectorDepositorInsertjAspSnFR3
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMO719
Plasmid#117479PurposeDesulfovibrio shuttle vector, contains pBG1 and pMB1 replicons, specRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Salmonella MTAN
Plasmid#64041PurposeExpression of S. enterica MTAN in E. coliDepositorInsertMethylthioadenosine nucleosidase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-FOXO3a WT
Plasmid#1790DepositorInsertFOXO3a (FOXO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYTK067
Plasmid#65174PurposeEncodes ConR1 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Dengue2 NS2B-NS3 proteins
Plasmid#204792PurposeBacterial expression of a fusion of Dengue2 NS2B-NS3 proteinsDepositorInsertDengue2 NS2B-NS3 fusion
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorUseTagsExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLlR5
Plasmid#149464PurposeBroad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human HUWE1 (3993-4374)
Plasmid#110754PurposeBacterial Expression of HUWE1 HECT domainDepositorInsertHUWE1 (HUWE1 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)
Plasmid#197662PurposeExpresses GFP under the control of J23100 and RiboJ-UTR1DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only