32,167 results
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Plasmid#128727PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertavrPtoB
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLE, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWPrinA-gfp2
Plasmid#123147PurposepSB1K3 carrying PrinA-30gfp-tDepositorInsertPrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBL713
Plasmid#140383PurposeExpresses CtcS protein under the control of T7-lacO promoterDepositorInsertT7-lacO-CtcS-T
Tags6XHisExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBK.134
Plasmid#187219PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectivelyDepositorInsertA: Retron Eco1 ncRNA v35 (long a1/a2); B: Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseSynthetic BiologyExpressionBacterialPromoterA: pTet*; B: pBetIAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1 Aquamarine
Plasmid#106285PurposeExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoterDepositorInsertAquamarine
Tags6xHis and TEV cleavage (N terminal on insert)ExpressionBacterialMutationT65S and H148GPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC3079 (p7INT-Pveg_lacI)
Plasmid#218505Purposeintegrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integraseDepositorAvailable SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD-eGFP
Plasmid#112918PurposeFluorescence microscopyDepositorInserteGFP
ExpressionBacterialPromoterPbadAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIK3C2GA
Plasmid#42451PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDSG318
Plasmid#115595Purposesurface-displayed Ag2, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
TagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCH1 - pBait-empty
Plasmid#174663Purposearabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
R701-X71-527: His6-tev-Hs.CALM1(1-80)
Plasmid#159694PurposeE. coli protein expression of His6-tev-Hs.CALM1(1-80)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
DDX20A
Plasmid#42376PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
Tags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagseGFPExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
Tags6X HisExpressionBacterialPromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
ExpressionBacterialMutationSUMO tag fused to N-terminus of PylBAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR_Lux
Plasmid#226457Purposelambda immunity region to the luciferase operon (lux)DepositorInsertlambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
ExpressionBacterialAvailable SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)
Plasmid#99224PurposeBacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).DepositorInsertpr-mEos3.2
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
k3(long) luciferase
Plasmid#11110DepositorAvailable SinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse islet1 [TJ#28]
Plasmid#16270DepositorInsertislet1 (Isl1 Mouse)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNR276
Plasmid#79577PurposeTP901 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR239
Plasmid#79576PurposeA118 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only