30,261 results

1. 4A3.PBAD-repL(AT2).J23115-gfp

   Plasmid

   #197873

   Purpose

   Arabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expression

   Depositor

   Baojun Wang

   Article

   Huan et al Front Microbiol. 2023 Feb 9;14:1095671.

   Insert

   part kilA/repL(AT2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO His6 SUMO SnRK2.6 (11-362)

   Plasmid

   #177847

   Purpose

   Bacterial Expression of SnRK2.6

   Depositor

   Karsten Melcher

   Article

   Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

   Insert

   SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT588

   Plasmid

   #68907

   Purpose

   NanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Insert

   NanoLuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV298

   Plasmid

   #204957

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and pyrG selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   pyrG
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PatoL-OG241

   Plasmid

   #72841

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PatoL

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST-p15a-pBAD-UTR1-TetR-T500

   Plasmid

   #45787

   Depositor

   Richard Murray

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-His6-ODC-Ctag

   Plasmid

   #163614

   Purpose

   Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   ODC (ODC1 Human)
   

   Use

   Tags

   C-tag and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSK-mPar1

   Plasmid

   #53227

   Purpose

   bacterial expression of mouse Par1

   Depositor

   Shaun Coughlin

   Insert

   Par1 (F2r Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pTriEx-mCherry-Zdk1-TIAM1 DH/PH

   Plasmid

   #81129

   Purpose

   mammalian expression of Zdk1 tagged Tiam1 Dh/PH

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   TIAM1 DH/PH (TIAM1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU

    Plasmid

    #64180

    Purpose

    harbours marker gene URA3

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY003 (pFnCpf1_delta Cas)

    Plasmid

    #69974

    Purpose

    Expresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus without Cas genes
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Cas genes are deleted from locus

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLRD14

    Plasmid

    #208266

    Purpose

    Production of syn-pimara-7,15-diene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    osCPS4
    osKSL4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Use

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Grp1-PH pGEX2TK

    Plasmid

    #71379

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pMAK461

    Plasmid

    #140699

    Purpose

    NBαRb-IgG-Fc

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαRb-IgG-Fc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDest-544

    Plasmid

    #11519

    Depositor

    Dominic Esposito

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    His6-NusA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge