30,807 results

1. pSHDY-PvanCC-mVenus_vanR

   Plasmid

   #137664

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the repressor vanR, as well as vanillate-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   PvanCC:mVenus
   

   Expression

   Bacterial

   Promoter

   PvanCC

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGM202T7

   Plasmid

   #69993

   Purpose

   E. coli/Streptomyces expression vector containing the T7-promoter

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aphII
   tsr
   rep
   sso
   T7 promoter
   dso
   His-Orf
   

   Tags

   N-terminal and C-terminal His tag encoding sequen…

   Expression

   Bacterial

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21b-P35

   Plasmid

   #11808

   Depositor

   Guy Salvesen

   Article

   Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

   Insert

   P35
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pSUB11

   Plasmid

   #169699

   Purpose

   Template plasmid for generating C-terminal 3xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   3xFLAG tag adjacent to FRT-flanked aph (KanR) gene
   

   Tags

   3xFLAG tag

   Expression

   Bacterial

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-tdTomato-hphMX6

   Plasmid

   #105157

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

   Insert

   tdTomato-hphMX6
   

   Tags

   tdTomato

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBJ4

   Plasmid

   #167133

   Purpose

   A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSB-wt

   Plasmid

   #197260

   Purpose

   wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

   Depositor

   Timothy Lohman

   Article

   Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

   Insert

   wt E. coli SSB
   

   Tags

   none

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SurA

   Plasmid

   #213138

   Purpose

   Expresses chaperone SurA

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   SurA (21-428) (surA E.coli)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLC-242

   Plasmid

   #73189

   Purpose

   Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

   Depositor

   Swaine Chen

   Article

   Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

   Insert

   chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
   

   Expression

   Bacterial

   Promoter

   chloramphenicol promoter

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Skp

    Plasmid

    #213139

    Purpose

    Expresses chaperone protein SKP

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SKP (21-161) (skp E.coli)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-MBP-huErCas12a

    Plasmid

    #174685

    Purpose

    Expresses 6xHis-MBP tagged ErCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huErCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis

    Expression

    Bacterial

    Mutation

    Q926R mutation is observed as indicated from the …

    Promoter

    T7

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. COMTD1

    Plasmid

    #25484

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COMTD1 (COMTD1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pTmpSm

    Plasmid

    #176228

    Purpose

    A template plasmid for amplification of the cI-hok-strA cassette

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

    Insert

    cI of phage lambda, hok of the R1 plasmid, strA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_RANBP2_Ran-BP2

    Plasmid

    #109839

    Purpose

    Protein expression and purification of RANBP2_Ran-BP2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RANBP2_Ran-BP2 (RANBP2 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BRD7 (BRD7A-c005)

    Plasmid

    #98245

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BRD7A (BRD7 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00439:Bromodomain 136-223

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLS.400

    Plasmid

    #187201

    Purpose

    Expresses retron Eco4 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco4 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    mphR

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBR322-Pdp1-HiBiT

    Plasmid

    #198310

    Purpose

    Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1-HiBiT
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-PFO

    Plasmid

    #92109

    Purpose

    Recombinant expression in E.Coli

    Depositor

    Feng Shao

    Article

    Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

    Insert

    PFO
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Delta27aa

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBP-Pspac

    Plasmid

    #112778

    Purpose

    Promoter repressed by LacI (not natively produced in B. subtilis 168)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    Pspac promoter
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_HSPA9_HSP70

    Plasmid

    #109776

    Purpose

    Protein expression and purification of HSPA9_HSP70

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    HSPA9_HSP70 (HSPA9 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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