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pCS2+ centrin-mKate2

Plasmid

#105941

Purpose

in vitro transcription, zf centrin N-terminally tagged with mkate2

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Centrin

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSC27

Plasmid

#117478

Purpose

mobilizable, SRB-stable, kanamycin-resistant cloning vector

Depositor

Judy Wall

Article

Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Rin1-SH2

Plasmid

#46474

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin1 (RIN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 54-204

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMZ0002

Plasmid

#102239

Purpose

Expresses Chinese hamster NSF in bacteria

Depositor

Axel Brunger

Article

Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

Insert

N-ethylmaleimide sensitive factor

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pETDuet1_CoiC_CoiSA

Plasmid

#208762

Purpose

Two coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiC
CoiSA

Expression

Bacterial

Mutation

single nucleotide insertion (C70) at RBS of CoiC

Promoter

T7

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T3-RhoA-Q63L

Plasmid

#12961

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Tags

GST

Expression

Bacterial

Mutation

Q63L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pHH0103_PRKACB_Pkinase

Plasmid

#110024

Purpose

Protein expression and purification of PRKACB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACB_Pkinase (PRKACB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSR1037

Plasmid

#208736

Purpose

Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PSarA1

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAB061i3

Plasmid

#79230

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ; PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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P22_wtCP_SP141his

Plasmid

#122651

Purpose

Expression of P22 virus like particle in bacteria

Depositor

Trevor Douglas

Insert

P22 Coat: WT / SP: 6xHis-SP141

Expression

Bacterial

Mutation

truncated SP, P120H in the capsid scaffold (see …

Available Since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAIP4_3KT9

Plasmid

#234192

Purpose

Heterologous protein expression of aprataxin in Escherichia coli

Depositor

Christopher Bahl

Insert

3KT9 (APTX Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSANG14-3F

Plasmid

#39265

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Tags

6xHis, Alkaline phosphatase, pelB leader, and tri…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pP450cam

Plasmid

#85085

Purpose

Expresses P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450cam

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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