30,261 results

1. pTSA-13

   Plasmid

   #17327

   Depositor

   Takeshi Sano

   Article

   Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

   Insert

   streptavidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated to include only amino acids 16-133 of m…

   Promoter

   Available since

   Feb. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Use

   Tags

   T120>A

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HLA-A2.1

   Plasmid

   #14904

   Depositor

   Linda Sherman

   Article

   Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

   Insert

   HLA-A2.1 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDFDuet-MKK6-EE

   Plasmid

   #82718

   Purpose

   Expresses constitutively active human MKK6. Multiple cloning site 2 is empty.

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

5. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKESa

   Plasmid

   #211828

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWPSG-Po-nCas9-BE

   Plasmid

   #195740

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Po (phoenol-inducible promoter)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BoNT/A-LC

   Plasmid

   #31602

   Depositor

   Axel Brunger

   Article

   Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

   Insert

   BoNT/A LC
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTDpelB-NTwinStrep

   Plasmid

   #45940

   Purpose

   use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Use

   Tags

   6xHis, PelB, and Twin-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Trc (lactose/IPTG inducible)

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPEPZ-Plac

    Plasmid

    #122635

    Purpose

    BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM33_Nsp4

    Plasmid

    #156474

    Purpose

    Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. JC Mad-1

    Plasmid

    #25626

    Depositor

    Peter Howley

    Article

    Howley et al J Virol. 1980 Dec . 36(3):878-82.

    Insert

    JC Virus Mad-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pET-X(5010)B1

    Plasmid

    #168019

    Purpose

    Expresses X(5010)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(5010)B1-6xHis
    

    Use

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Reb_1 aka PAS619

    Plasmid

    #71224

    Purpose

    Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA G53A

    Promoter

    T7

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

16. OTUB1

    Plasmid

    #25149

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    otub1.040.271 (OTUB1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acid residues 40-271

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pGEMTEZ-Kir2.1

    Plasmid

    #32641

    Depositor

    Richard Axel, Joseph Gogos, C. Ron Yu

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Kir2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDDYFP-B

    Plasmid

    #40289

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    ddYFP-B
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid

    #52054

    Purpose

    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)

    Depositor

    Jesse Rinehart

    Inserts

    SepRS
    EF-Sep
    5x tRNA Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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