30,807 results

1. pH6HTC_IRP1-Halo

   Plasmid

   #175305

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   IRP1 (ACO1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-29b(+)-mSandy2

   Plasmid

   #177760

   Purpose

   Expresses mSandy2 using the T7 Promoter

   Depositor

   Roberto Chica

   Article

   Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

   Insert

   mSandy2
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pspCas9-Klenow-ST2-com-vector

   Plasmid

   #176235

   Purpose

   Vector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.

   Depositor

   Baisong Lu

   Article

   Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-2(KW)2-mCherry

   Plasmid

   #112738

   Purpose

   Express 2(KW)2-mCherry in E. coli

   Depositor

   Kyubong Jo

   Article

   Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

   Insert

   2(KW)2-mCherry
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest-544

   Plasmid

   #11519

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   His6-NusA

   Expression

   Bacterial

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNAS1B

   Plasmid

   #61968

   Purpose

   Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   mCherry
   GFP
   

   Expression

   Bacterial

   Mutation

   Serine 65 to Glycine (GGT), Serine 72 to Alanine …

   Promoter

   PBAD and PLtetO

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

   Plasmid

   #238003

   Purpose

   Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Inserts

   AtRBX1 (RBX1 Mustard Weed)
   AtSKP1 (SKP1 Mustard Weed)
   AtCUL1 (CUL1 Mustard Weed)
   

   Tags

   S and T7

   Expression

   Bacterial

   Available Since

   June 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYO1125

   Plasmid

   #235742

   Purpose

   Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   p40 PX domain (NCF4 Human)
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   aa 2-149 with Cys at 2

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-MjCouRS

   Plasmid

   #229066

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

   Insert

   Methanococcus jannaschii tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGal-Trans-Myc-eGFP-KDEL

    Plasmid

    #177729

    Purpose

    ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    

    Tags

    KDEL, Myc, and eGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. R701-X72-527: His6-tev-Hs.CALM1(76-148)

    Plasmid

    #159695

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(76-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(76-148) (CALM1 Human)
    

    Tags

    His6-tev

    Expression

    Bacterial

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHHM.X513-iNicSnFR3b-(V7)

    Plasmid

    #124881

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

    Depositor

    Henry Lester

    Article

    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

    Insert

    iNicSnFR3b-(V7)
    

    Tags

    HA Tag, His Tag, and NA

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    T7

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-beta-arrestin2

    Plasmid

    #36919

    Depositor

    Robert Lefkowitz

    Article

    Xiao et al J Biol Chem. 2004 Dec 31;279(53):55744-5

    Insert

    beta-arrestin2 (Arrb2 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-EPN-24

    Plasmid

    #79939

    Purpose

    PH-Late1-I3-01-myc

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-24
    

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHBFD1Bad

    Plasmid

    #53564

    Purpose

    Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

    Insert

    hbfd1 cDNA
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLC-284

    Plasmid

    #124294

    Purpose

    A low copy plasmid constitutively expressing vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.

    Depositor

    Swaine Chen

    Article

    Eshaghi et al Pathogens. 2016 Jan 5;5(1). pii: pathoge

    Insert

    vsfGFP-9
    

    Expression

    Bacterial

    Available Since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME Integrin beta 1b

    Plasmid

    #105976

    Purpose

    entry clone for gateway cloning, Full length Integrin beta 1b without stop

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Integrinbeta1b
    

    Expression

    Bacterial

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. His_CL7_2NLS_iCas12a-4NLS

    Plasmid

    #222971

    Purpose

    Bacterial expression of NLS-rich iCas12a construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

    Insert

    His_CL7_2NLS_iCas12a-4NLS
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. N-WASP

    Plasmid

    #186132

    Purpose

    For expression of recombinant N-WASp in bacteria cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    WASL (WASL Rat)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Amino Acid residues 183-193 fused to 273-501

    Promoter

    T7

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Tags

    6XHIS tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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