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pTHSSe_45

Plasmid

#109243

Purpose

pSC101 var3 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSW002

Plasmid

#111248

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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S100A6

Plasmid

#196217

Purpose

Codon-optimized bacterial expression plasmid. Expresses S100A6.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

S100A6 (S100A6 Human)

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pccGFPKAN

Plasmid

#73649

Purpose

Fluorescent reporter for transmembrane protein dimerization.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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KSP_K406

Plasmid

#129771

Purpose

Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays

Depositor

William Hancock

Insert

KSP (kif11.L Frog)

Tags

His6

Expression

Bacterial

Mutation

Truncation at 367, fused to kinesin-1 starting Al…

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid

#176742

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FXR1P

Plasmid

#198694

Purpose

Expression of human FXR1 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR1P (FXR1 Human)

Tags

MBP

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-hcGAS(157-522)

Plasmid

#108676

Purpose

For expression in E.coli and affinity purification of N-terminally GST-tagged human cGAS amino acid 157-522

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

cGAS amino acid 157-522 (CGAS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acid 157-522

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a T7pCONS sfGFP

Plasmid

#154462

Purpose

pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-MjCouRS

Plasmid

#229066

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

Methanococcus jannaschii tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-mNectarine

Plasmid

#21717

Purpose

Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

Depositor

Robert Campbell

Article

Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

Insert

mNectarine fluorescent proteins

Tags

His

Expression

Bacterial

Mutation

The fluorescent protein was inserted between XhoI…

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pRMCD28

Plasmid

#167556

Purpose

Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

Type

Empty backbone

Tags

PhoA'

Expression

Bacterial

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP2a-Halo

Plasmid

#175309

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Article

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p2.0VPI.EGFP

Plasmid

#40868

Depositor

Harold Gainer

Article

Ponzio et al PLoS One. 2012;7(11):e48860. doi: 10.137

Insert

mouse vasopressin promoter

Use

AAV

Expression

Bacterial and Insect

Promoter

mouse vasopressin

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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MSP1673

Plasmid

#65769

Purpose

Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pTECH-MbPylRS(IPYE)

Plasmid

#104072

Purpose

Expression plasmid encoding MbPylRS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MbPylRS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12i1

Plasmid

#120882

Purpose

Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12i1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Expression

Bacterial

Promoter

gpdA;trpC

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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