30,807 results

1. APRT

   Plasmid

   #64028

   Purpose

   Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.

   Depositor

   Vern Schramm

   Article

   Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9

   Insert

   adenine phosphoribosyltransferase (APT1 Budding Yeast)
   

   Tags

   Histidine x 6

   Expression

   Bacterial

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCas-MmdCas12m-CBE1

   Plasmid

   #192284

   Purpose

   MmdCas12m-CBE1 expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmdCas12m-CDA-UGI
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D485A

   Promoter

   PJ23108

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTE100

   Plasmid

   #59395

   Purpose

   MethyloBrick cloning vector, no promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116954

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-link-sfGFP-FLAG:Kan

   Plasmid

   #211833

   Purpose

   Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   sfGFP-FLAG-Kan
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p15aC-1D

   Plasmid

   #107863

   Purpose

   Bacterial expression of PIS

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   PIS
   

   Tags

   myc

   Expression

   Bacterial

   Promoter

   proD

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDDYFP-B

   Plasmid

   #40289

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   ddYFP-B
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPSV39-ugi

    Plasmid

    #186556

    Purpose

    Broad host range inducible expression of uracil-DNA glycosylase inhibitor (UGI)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    ugi
    

    Expression

    Bacterial

    Promoter

    LacUV5

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-5X2-RalBP1

    Plasmid

    #37245

    Depositor

    Christopher Counter, David Kashatus

    Article

    Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

    Insert

    RalBP1 (RALBP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET28CLY9

    Plasmid

    #21769

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pMycVec1

    Plasmid

    #32360

    Depositor

    Michael Niederweis

    Article

    Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

    Insert

    ColE1 origin replication
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGex-4T-2_GABARAP

    Plasmid

    #73948

    Purpose

    For recombinant expression of human GABARAP in E.coli

    Depositor

    Dieter Willbold

    Article

    Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

    Insert

    GABARAP (GABARAP Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-T-rnVAPA-1-125

    Plasmid

    #13396

    Depositor

    Axel Brunger

    Article

    Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

    Insert

    VAP-A (Vapa Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

16. p405TEF1

    Plasmid

    #15968

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    TEF1 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD-PSmOrange2

    Plasmid

    #34957

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2012 Aug 17.

    Insert

    PSmOrange2
    

    Expression

    Bacterial

    Mutation

    R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTDpelB-NTwinStrep

    Plasmid

    #45940

    Purpose

    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis, PelB, and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPS-7942

    Plasmid

    #80459

    Purpose

    IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

    Depositor

    Daniel Ducat

    Article

    Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

    Insert

    Sucrose phosphate synthase
    

    Expression

    Bacterial

    Available Since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Expression

    Bacterial

    Promoter

    PR promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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