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pGEX6P1-NEDD4L_FL

Plasmid

#187712

Purpose

Express GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV109

Plasmid

#215339

Purpose

Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PCin

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pPJ23116-MS2-rep

Plasmid

#184842

Purpose

Expression of the rep protein of phage MS2

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Replicase gene MS2 phage

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTXB1.Tat(48-57)-Cre recombinase

Plasmid

#165042

Purpose

Expression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

Cre recombinase

Tags

Mxe Intein-Chitin binding domain and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYSD085

Plasmid

#212922

Purpose

Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

649 Stalk

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lsh_C2c2_WT

Plasmid

#83487

Purpose

Bacterial expression of WT L. shahii C2c2 CRISPR effector

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lsh_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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TS246_pNKI-2-13_HALO-DPN7

Plasmid

#200720

Purpose

producing Methyl Tracer HALO protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

HALO-DPN7

Tags

6xHis

Expression

Bacterial

Mutation

codon optimized

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-PSmOrange2

Plasmid

#34957

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2012 Aug 17.

Insert

PSmOrange2

Expression

Bacterial

Mutation

R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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6798 pJC25NMstop

Plasmid

#1228

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

NM (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-tdTomato-hphMX6

Plasmid

#105157

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Zhu et al Mol Biol Cell. 2013 Oct;24(20):3187-204.

Insert

tdTomato-hphMX6

Tags

tdTomato

Expression

Bacterial and Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJBL723

Plasmid

#140393

Purpose

Expresses HucR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-HucR-T

Tags

6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJKR-H-cdaR

Plasmid

#62557

Purpose

Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Inserts

beta lactamase
sfGFP
CdaR

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-mLemon

Plasmid

#191256

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein mLemon

Depositor

Robert Campbell

Insert

mLemon

Tags

His tag

Expression

Bacterial

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12a

Plasmid

#164658

Purpose

Bacterial expression of LbCas12a using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT217

Plasmid

#134885

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET-15b PAI-RBP

Plasmid

#45390

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP (SERBP1 Human)

Tags

His

Expression

Bacterial

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only