30,249 results

1. pFLP3

   Plasmid

   #64946

   Purpose

   Flp recombinase expression vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   Flp recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lambda promoter/temperature-sensitive Lambda cI r…

   Available since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pRE112

   Plasmid

   #43828

   Depositor

   Dieter Schifferli

   Article

   Edwards et al Gene. 1998 Jan 30;207(2):149-57.

   Type

   Empty backbone

   Use

   Bacterial allelic exchange vector with sacb1

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-TtCas6-4xcrRNA4.5

   Plasmid

   #127764

   Purpose

   For expression of T. thermophilus Csm complex in E. coli (crRNA)

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

   Inserts

   Synthetic CRISPR array containing four identical T. thermophilus spacers
   Cas6A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCold-I-Dsup

   Plasmid

   #90021

   Purpose

   To express His-tagged Dsup in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBZS-M.CviPII (NDel15)

    Plasmid

    #198356

    Purpose

    Express M.CviPII (NDel15) in bacterial cells.

    Depositor

    Takashi Ito

    Article

    Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

    Insert

    M.CviPII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB1-moxCerulean3

    Plasmid

    #103975

    Purpose

    Plasmid encoding moxCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxCerulean3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSKB3.MPN348

    Plasmid

    #11437

    Depositor

    Sung-Hou Kim

    Article

    Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

    Insert

    methenyltetrahydrofolate synthetase
    

    Use

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28A-Rab8A

    Plasmid

    #228880

    Purpose

    Expression of His-tagged Rab8A in E. coli

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    residues 1-6 and 177-207 missing

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-SNAP-PI(3,5)P2 Probe

    Plasmid

    #211512

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SnxA-2xPX
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-19b_MMLV-RT

    Plasmid

    #153312

    Purpose

    Bacterial expression of MMLV reverse transcriptase

    Depositor

    Drew Endy, Philippa Marrack

    Insert

    MMLV Reverse Transcriptase
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCRISPomyces-2

    Plasmid

    #61737

    Purpose

    Streptomyces expression of codon-optimized Cas9 and custom gRNA

    Depositor

    Huimin Zhao

    Article

    Cobb et al ACS Synth Biol. 2014 Dec 8.

    Inserts

    sSpCas9
    gRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    BbsI-flanked lacZ cassette inserted in place of s…

    Promoter

    gapdhp(EL) and rpsL(XC)-BbsI

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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