31,059 results
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Plasmid#44182DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only
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pFW2500
Plasmid#224213PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1 and recTDepositorInsertFncpf1 and recT
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pcr4-12xMBS-PBS
Plasmid#52984Purposeprovide MBS-PBS alternating sequenceDepositorInsert12xMBS-PBS
ExpressionBacterialPromoternoneAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
p27-mVenus reporter (PB)
Plasmid#190731PurposeThe p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.DepositorInsertsmVenus
p27
ExpressionBacterial and MammalianMutationp27-F62A-F64APromoterEF1αAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
Tags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-T-GECO1
Plasmid#191616PurposeBacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1DepositorInsertT-GECO1
TagsHis tagExpressionBacterialAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24b-APEX2
Plasmid#111702PurposeFor IPTG-inducible expression of APEX2 in E. coliDepositorInsertAPEX2
Tags6xHis and V5ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-2
Plasmid#61737PurposeStreptomyces expression of codon-optimized Cas9 and custom gRNADepositorInsertssSpCas9
gRNA cassette
UseCRISPRExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL) and rpsL(XC)-BbsIAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-aHL_spytag_his
Plasmid#107334PurposeMonomeric SpyTag-6×-His alpha-hemolysinDepositorInsertalpha-hemolysin-SpyTag-6xHis
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-EGFP-CNA35
Plasmid#61603Purposebacterial expression of N-terminal fusion protein of CNA35 with EGFPDepositorInsertEGFP and CNA35
TagsHis tagsExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-DmPABPC1_J
Plasmid#146647PurposeBacterial Expression of DmPABPC1DepositorAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
TagsHis6x tagExpressionBacterialPromoterT7Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQmod2S-GG
Plasmid#191346PurposeClostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRKH3-slpGFP
Plasmid#27168DepositorInsertsurface (S)-layer protein promoter region fused with modified green fluorescent protein 5
ExpressionBacterialAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
HP-Cas9hGem-Ade2-LEU2-LINEAR
Plasmid#174839PurposepRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorphaDepositorInsertHH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoterTDH3pAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneExpressionBacterialPromoterJ231196Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3
Plasmid#47067PurposeFX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only