32,069 results
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Plasmid#194211PurposeCJ8421_04975 Rptr2 targeted DNA sequenceDepositorInsertCJ8421_04975 Rptr2 DNA target
ExpressionBacterialAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
ExpressionBacterialPromoterlac UV5Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP4
Plasmid#129507PurposeExpresses AvicFP4 constitutively in E. coli (most strains)DepositorInsertAvicFP4
ExpressionBacterialMutationOne of two variants of AvicFP4 tested with essent…Promotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cdk9 T186A
Plasmid#28101DepositorInsertCdk9 (CDK9 Human)
TagsFLAGExpressionBacterial and MammalianMutationContains a T186A mutation in Cdk9Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ACPs (from S. pneumonia)
Plasmid#63687PurposeInducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniaeDepositorInsertacpS
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-IkappaBalpha(FL)
Plasmid#44738DepositorAvailable SinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEC3006 (pSpy1C-PZn_ffluc)
Plasmid#218515Purposereplicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenesDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of Plac_mrfp cassettePromoterPZn (PczcD)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS:TVA-mCherry
Plasmid#187823PurposeExpression of a fusion of the transmembrane protein TVA and the red fluorescent protein mCherry under UAS control.DepositorInsertTVA-mCherry
ExpressionBacterialPromoter5xUASAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1415
Plasmid#84828PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-recAmu-sacB
Plasmid#158717PurposeCRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.DepositorInsertrecA
ExpressionBacterialMutationR61CAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYB3-PUM1-HD
Plasmid#17543DepositorAvailable SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDSG310
Plasmid#115611Purposesurface-displayed Ag2, Arabinose-inducible; medium-copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-gst-xr
Plasmid#214761PurposeXylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coliDepositorInsertglutathione S-transferases fused with xylose reductase
TagsGlutathione S-transferasesExpressionBacterialPromoterT7Available SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-GST-CD(Cbx7)
Plasmid#82525PurposeExpresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 chromodomain only; amino acids 1–62.DepositorInsertChromobox Homolog 7 (CBX7 Human)
TagsGSTExpressionBacterialMutationCD(Cbx7) ; amino acids 1–62Available SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SUMO1
Plasmid#185637Purposeexpression of GST-tagged SUMO1 in bacterial cellsDepositorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1MycHis/HIP1
Plasmid#29590DepositorAvailable SinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB
Plasmid#26607DepositorAvailable SinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only