30,307 results
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Plasmid#47802PurposePesaR promoter, luxCDABE, and terminator in pET17b (AmpR)DepositorInsertPesaR-luxCDABE-term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPesaRAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorInsertAcpP (acpP E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINVL
Plasmid#26040DepositorInsertu-LacZ
UseTagsu-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYTK076
Plasmid#65183PurposeEncodes HIS3 as a Type 6 part to be used in the Dueber YTK systemDepositorInsertHIS3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorInsertSAHS1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorInsertCRYAA (CRYAA Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR4pemIK-GW
Plasmid#126929PurposeBroad host range vector with toxin-antitoxin stability system for maintenance in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2
Plasmid#133013PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
UseTags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationPromotert7Available sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoterAraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-8
Plasmid#172772PurposeBacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorInsertIL37 (IL37 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorInsertHRASLS3 (PLAAT3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag
Plasmid#187356PurposeBacterial expression and refolding of gp100 pMHCDepositorInserthHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)_beta2M (MHC Class I light)
Plasmid#187357PurposeBacterial expression and refolding of gp100 pMHCDepositorInsertbeta2M (MHC Class I light)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only