30,808 results

1. Nano-lantern/pRSETB

   Plasmid

   #51969

   Purpose

   bacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Available Since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP3654

   Plasmid

   #222349

   Purpose

   Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

   Depositor

   Jason Peters

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PabstBR

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEK36

   Plasmid

   #210598

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
   

   Tags

   mClover3

   Expression

   Bacterial and Yeast

   Promoter

   SpH2Bpro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RAB5AA-c003

   Plasmid

   #175475

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-N215.

   Depositor

   Opher Gileadi

   Insert

   RAB5A (RAB5A Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SENP7 (catalytic domain)

   Plasmid

   #16360

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP7 catalytic domain (SENP7 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino Acid 640 to 984 catalytic domain of human S…

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pMYO

   Plasmid

   #34626

   Depositor

   Jesse Rinehart, Dieter Söll

   Article

   Park et al Science. 2011 Aug 26;333(6046):1151-4.

   Insert

   Myoglobin
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   lpp

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24a MDH_AQUAR

   Plasmid

   #204239

   Purpose

   Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_AQUAR
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGEX3X-GST-PTEN

   Plasmid

   #20743

   Depositor

   Alonzo Ross

   Article

   Campbell et al J Biol Chem. 2003 Sep 5. 278(36):33617-2

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pDonor14-ShoHELIX-KanR (BO4)

   Plasmid

   #181795

   Purpose

   pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Expression

    Bacterial

    Promoter

    araBAD promoter

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEND-400_Cacnes_CAMP2

    Plasmid

    #225606

    Purpose

    Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB602-RBCS-tagRFP

    Plasmid

    #213868

    Purpose

    Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

    Insert

    RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
    

    Expression

    Bacterial and Plant

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWB508-cTP-mCherry

    Plasmid

    #213862

    Purpose

    Binary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Expression

    Bacterial and Plant

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pED14xS-4

    Plasmid

    #134802

    Purpose

    IPTG inducible expression of S-TAGA-Evo3 qtRNA

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    S-TAGA-Evo3 qtRNA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn5-140

    Plasmid

    #118524

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid

    #60865

    Purpose

    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C

    Depositor

    Hermann Gaub

    Article

    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

    Inserts

    Xyl
    Xmod-DocIII
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Threonine 120 to Cysteine in Xylanase domain

    Promoter

    T7

    Available Since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP84

    Plasmid

    #86554

    Purpose

    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile

    Depositor

    Wiep Klaas Smits

    Insert

    Pveg-sLucopt
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pveg

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MP3

    Plasmid

    #69650

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam
    

    Expression

    Bacterial

    Promoter

    E. coli PBAD

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM1s3AsR_TS-C7

    Plasmid

    #137945

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Insert

    mRFP1
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Expression

    Bacterial

    Promoter

    J23101

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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