32,167 results
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Plasmid#226654PurposepET24b-p97-FLAGDepositorAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAU171
Plasmid#86835Purposebacterial expression of anti-CRISPR protein AcrIIA2DepositorInsertacrIIA2
ExpressionBacterialPromoteraraBADAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
RAB5B
Plasmid#25251PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB5B:A15-G191 (RAB5B Human)
TagsHisExpressionBacterialMutationContains amino acids A15-G191Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
ZIKV_Sensor_32B_LacZ
Plasmid#75007PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_32B_LacZ
TagsLacZExpressionBacterialPromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originExpressionBacterialAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
Tags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIB168
Plasmid#90191PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-TEV-pro-conA
Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
TagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialPromoterT5Available SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-rSyntenin1
Plasmid#89428Purposebacterial expression of rat Syntenin1DepositorInsertSDCBP
TagsHisExpressionBacterialPromoterT5Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorAvailable SinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
ExpressionBacterialAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPath-pTac-integrase
Plasmid#199999PurposeEntry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.DepositorInsertPathways
ExpressionBacterialAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(36-235)
Plasmid#170533PurposeExpresses Cleavable His-tagged PTPIP51_ΔTPR domain (36-235) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsHis tagExpressionBacterialPromoterT5 promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-FOXP2_P6
Plasmid#122649PurposeExpresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cellsDepositorInsertHomo sapiens forkhead box P2 (FOXP2 Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7Available SinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-Ppuf843-1200-PR
Plasmid#50963PurposeExpresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroidesDepositorInsertProteorhodopsin
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterPpuf843-1200Available SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…ExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet28a-ClbB-NRPS-NHis
Plasmid#49217PurposeNRPS module of ClbB containing CAT domains with N-terminal 6His tagDepositorInsertClbB-NRPS
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa4-1080 of reference sequence NP_754362…PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTE-mcs
Plasmid#20320DepositorInsertmultiple cloning site
ExpressionBacterialAvailable SinceMarch 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-hsGFP
Plasmid#64911PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in E. ColiDepositorInserthsGFP
TagsHis TagExpressionBacterialMutationChromophore tyrosine of the protein mutated to TA…PromoteraraBADAvailable SinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB3B (3DZ8)
Plasmid#32879PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB3B (RAB3B Human)
TagsHis tag (with TEV cleavage)ExpressionBacterialMutationamino acids 18-190 onlyAvailable SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-yibDp-Erv1p
Plasmid#212094PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-EV
Plasmid#212095Purpose(Empty Backbone) pCOLA-Spec Empty VectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only