30,808 results

1. pBEST-p15a-pBAD-UTR1-TetR-T500

   Plasmid

   #45787

   Depositor

   Richard Murray

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-SPA2G231V

   Plasmid

   #42079

   Depositor

   Christine Garcia

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   Surfactant Protein -A2 (SFTPA2 Human)
   

   Use

   Lentiviral; Plenti

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Changed Glycine 231 to Valine

   Promoter

   CMV

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR8/GW/TOPO PCGF2

   Plasmid

   #69791

   Purpose

   Gateway entry vector for PCGF2

   Depositor

   Gerard Cagney

   Insert

   PCGF2 (PCGF2 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCN38-yfp

   Plasmid

   #227637

   Purpose

   Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

   Depositor

   Angelika Grundling

   Article

   Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

   Insert

   yfp
   

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MP-P

   Plasmid

   #69661

   Purpose

   Untargetted Bacterial Mutagenesis

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR mutH(E56A)
   

   Expression

   Bacterial

   Promoter

   E. coli PBAD

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Expression

   Bacterial

   Promoter

   Pdhfr

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_TXK-1/1

   Plasmid

   #91539

   Purpose

   Protein expression and purification of human SH3 domain construct TXK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TXK-1/1 (TXK Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103_TEC-1/1

   Plasmid

   #91252

   Purpose

   Protein expression and purification of human SH3 domain construct TEC-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TEC-1/1 (TEC Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_ITK-1/1

    Plasmid

    #91253

    Purpose

    Protein expression and purification of human SH3 domain construct ITK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ITK-1/1 (ITK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-X0

    Plasmid

    #115565

    Purpose

    Control vector expressing GFP alone without any consensus repeats

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Expression

    Bacterial and Yeast

    Promoter

    ADH1

    Available Since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. AdML-M3

    Plasmid

    #11244

    Depositor

    Robin Reed

    Article

    Zhou et al Proc Natl Acad Sci U S A. 2002 Sep 17. 9

    Insert

    AdML-M3
    

    Tags

    3 MS2 hairpins

    Expression

    Bacterial

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pGBW-m4046852

    Plasmid

    #145584

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp8

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMAL-c5X-MAP

    Plasmid

    #66999

    Purpose

    Expresses malE-MAP in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-MAP
    

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX 6P1 Cyclin A (555)

    Plasmid

    #61844

    Purpose

    bacterial expression of GST cyclin A

    Depositor

    Jonathon Pines

    Insert

    cyclin A (CCNA2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L2V

    Promoter

    tac

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-PesaRlux

    Plasmid

    #47802

    Purpose

    PesaR promoter, luxCDABE, and terminator in pET17b (AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-luxCDABE-term
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PesaR

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pET21b-Tipin-His

    Plasmid

    #23122

    Depositor

    Aziz Sancar

    Article

    Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

    Insert

    Tipin (TIPIN Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pJMP8823

    Plasmid

    #220901

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mucin MUC1-5TR-pET15b

    Plasmid

    #174694

    Purpose

    Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

    Depositor

    Jean Gariepy

    Article

    Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

    Insert

    MUC1-TR
    

    Tags

    hexahistidine (HIS) tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7 Promoter

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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