32,167 results
-
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only
-
PTPRK
Plasmid#38968PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBW400hrpL-gfp
Plasmid#61437PurposepBW400hrpL-gfpDepositorInserthrpL-rbs30-gfp-ter
ExpressionBacterialPromoterHrpLAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTSSX-STC
Plasmid#115668PurposeE.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tagDepositorInsertsmall tetraheme cytochrome c
TagsSignal peptide (aa1-27)-Strep-II tag- Factor Xa c…ExpressionBacterialPromotertacAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
EHF_pet28a
Plasmid#131660PurposeBacterial expression of ETS gene EHFDepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC012 LwCas13a locus with nontargeting spacer
Plasmid#91904PurposeLwCas13a locus into pACYC184 with nontargeting spacerDepositorInsertLwCas13a with non-targeting spacer
UseCRISPRExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSARSF53-110
Plasmid#61813PurposeTvoVMAN11 inteinDepositorInsertTvoVMAN11 intein
TagsH6-Smt3ExpressionBacterialPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-98
Plasmid#61827PurposeTvoVMAdN11 inteinDepositorInsertTvoVMAdN11 intein
TagsGB1-H6ExpressionBacterialPromoterpBADAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasyG1_hph
Plasmid#184912PurposeTemplate to generate via PCR a single gRNA for expression in S. cerevisiaeDepositorInsertgRNA scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_DARPinOFF7
Plasmid#182396PurposeBacterial expression of DARPinOFF7DepositorInsertDARPinOFF7
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HS-OS-Plk4-mKate2
Plasmid#105944Purposeheat shock inducible transgenesis, non phoshorylable version of Plk4DepositorInsertPlk4 (Plk4 Mouse)
TagsmKate2ExpressionBacterialMutation1002 to 3704 of Plk4/ deletion of S282-S305.Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
DYRK2
Plasmid#39031PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL630
Plasmid#167836PurposeEncodes OptoLAC3 circuitDepositorInsertlacI
UseSynthetic BiologyTagsssrA (AAV)ExpressionBacterialAvailable SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMT-4
Plasmid#22837DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
ExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046795
Plasmid#145598PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBbS7k-RFP
Plasmid#35321DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
PE_26: Para_Lac_Tet_eG::sfYFP
Plasmid#104887PurposePE_26: Para_Lac_Tet_eG::sfYFPDepositorInsertHybrid Promoter Para_Lac_Tet_eG
ExpressionBacterialPromoterPara_Lac_Tet_eGAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-IP2.0
Plasmid#109217PurposeExpress IP2.0 in E. coliDepositorInsertIP2.0 (Inverse Pericam 2.0)
TagsHis-tagExpressionBacterialAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAF1
Plasmid#39118PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
p109.1
Plasmid#44518DepositorInsertHistone 4
TagsGSTExpressionBacterialMutationcontains full length (aa 1-102)Available SinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
TagsGFPExpressionBacterial and YeastPromoterSpunHsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1R1
Plasmid#97323PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1R1
Tags6xHis - TEVExpressionBacterialMutationAddition of R residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2
Plasmid#124218PurposeCytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2DepositorInsertmNG-sfTq2
ExpressionBacterialAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
ExpressionBacterialPromoterPBAD and PLtetOAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAE03
Plasmid#61044PurposeTo construct C-terminal GFP fusions, single crossover integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsGFP+ExpressionBacterialAvailable SinceFeb. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -