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32,838 results

Showing: 10081 - 10110 of 32838 results

  2. pETM-60-NusA-DmBelle_B

    Plasmid
    #145963
    Purpose
    Bacterial Expression of DmBelle
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle (bel Fly)
    Expression
    Bacterial
    Mutation
    del 155-157, T530A and I685V
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    145963-plasmid-map-sequence-id-333991

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  3. pKB224

    Plasmid
    #170014
    Purpose
    Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    NC
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170014-plasmid-map-sequence-id-335947

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  5. pGBK CUP1 EGFP

    Plasmid
    #170210
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    enhanced green fluorescent protein
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Promoter
    CUP1
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170210-plasmid-map-sequence-id-335104

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  6. pKB233

    Plasmid
    #170040
    Purpose
    Encodes PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170040-plasmid-map-sequence-id-336782

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  16. pTJV1Sc3-rpoB

    Plasmid
    #166983
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    RecT (recT E. coli)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVvanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166983-plasmid-map-sequence-id-331334

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  17. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  22. FEM1C

    Plasmid
    #167842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167842-plasmid-map-sequence-id-331056

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  24. L-RARS-5

    Plasmid
    #166535
    Purpose
    scFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166535-plasmid-map-sequence-id-329207

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  25. L-KARS-5

    Plasmid
    #166530
    Purpose
    scFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166530-plasmid-map-sequence-id-329204

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  29. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  30. t1-405

    Plasmid
    #166127
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    Tln1Head (1-405) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405 only
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166127-plasmid-map-sequence-id-330252

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Showing: 10081 - 10110 of 32838 results