30,808 results

1. Ubiquitin K63C

   Plasmid

   #86589

   Purpose

   Bacterial expression of Ubiquitin K63C for cross linking studies

   Depositor

   Sharon Rozovsky

   Article

   Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

   Insert

   Ubiquitin (UBC Human)
   

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   TEV site; K63C

   Promoter

   Tac

   Available Since

   Feb. 24, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. H6hSDHA

   Plasmid

   #113045

   Purpose

   Hetero expression of His-tagged human SDHA

   Depositor

   Edward A. Berry

   Article

   Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

   Insert

   H6hSDHA (SDHA Human)
   

   Tags

   His6 (MGSSHHHHHHSQDP-)

   Expression

   Bacterial

   Mutation

   Insert begins at amino acid 43

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmRE-Tn5-147

   Plasmid

   #118526

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-C2m2-mKate

   Plasmid

   #208765

   Purpose

   To express C2m2-mKate in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-mKate (Mfge8 Mouse)
   

   Tags

   mKate

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4R3H

   Plasmid

   #64652

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available Since

   April 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pCS2 PCNA GFP

   Plasmid

   #105937

   Purpose

   in vitro transcription, cell cycle nuclear marker

   Depositor

   Caren Norden

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   PCNA (PCNA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSara-C

   Plasmid

   #60722

   Purpose

   Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 180-880 of T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Only amino acids 180-880 of T7 RNAP

   Promoter

   PBAD

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSara-N

   Plasmid

   #60721

   Purpose

   Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   Residues 1-179 of T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Only amino acids 1-179 of T7 RNAP

   Promoter

   PBAD

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonorCm(Ptr)

    Plasmid

    #175577

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    Mini-Tn, Cm cassette
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLC-238

    Plasmid

    #73192

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-chpB (chpB )
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. ASB9A-c009

    Plasmid

    #53670

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    ASB9A
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

    Plasmid

    #29723

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mO…

    Expression

    Bacterial

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

    Plasmid

    #153527

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#68

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#68
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Csy4H29AS50C

    Plasmid

    #45029

    Depositor

    Jennifer Doudna

    Article

    Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

    Insert

    Csy4H29AS50C
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    H29A mutation to abolish catalytic activity and S…

    Promoter

    T7

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pET mCitrine LIC cloning vector (u-mCitrine)

    Plasmid

    #29771

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mCitrine

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. 8 pCOLI_A_GST

    Plasmid

    #160150

    Purpose

    N-term GST 3C cleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSNAP-CLC-EYFP

    Plasmid

    #38011

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Tags

    EYFP and SNAP26m

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYC1446

    Plasmid

    #158720

    Purpose

    Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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