31,065 results
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Plasmid#175356PurposeSupport plasmid for T7_ori plasmidsDepositorInsertT7RNAP-Q737P
ExpressionBacterialMutationQ737PAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDule-Mb haloTyrRS C6
Plasmid#160377PurposeC6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.DepositorInsertC6 HaloTyrosine tRNA synthatase
UseSynthetic BiologyExpressionBacterial and MammalianMutationL270S, Y271L, N311G, C313TAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTKIP-neo
Plasmid#41064DepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
ExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CTBP2
Plasmid#38799PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC133
Plasmid#198639PurposeExpression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.DepositorInsertdCas9
Tags3x-FLAGExpressionBacterialPromoterpTetAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840AAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
enz.083-NMT1
Plasmid#154047PurposeExpresses NMT1 in E. coliDepositorAvailable SinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
TagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSHS212
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
TagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialPromotertac promoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrc99A-Rat FABP1
Plasmid#13577DepositorInsertRat liver fatty acid binding protein (Fabp1 Rat)
ExpressionBacterialAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
human MTAP
Plasmid#64077PurposeExpression of human 5'-methylthioadenosine phosphorylaseDepositorInsert5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
TagsHistidine tag and TEVExpressionBacterialAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 WT
Plasmid#8594DepositorAvailable SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
RALGDS
Plasmid#25331PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRALGDS:N793-F914 (RALGDS Human)
TagsHisExpressionBacterialMutationContains amino acids N793-F914Available SinceSept. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-30 Ubwt (2)
Plasmid#26685DepositorInsertUbiquitin
TagsHis and S-tagExpressionBacterialAvailable SinceNov. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAT588
Plasmid#68907PurposeNanoLuc expressed constiutively from PBT1311 and GH023 RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only