30,327 results

1. pEF019 MBP-β-catenin-His

   Plasmid

   #154070

   Purpose

   Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b/bPanK

   Plasmid

   #73619

   Purpose

   Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

   Depositor

   Suzanne Jackowski, T. J. Kappock

   Article

   Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

   Insert

   pantothenate kinase (coaA Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   relative to MG1655 sequence, changes glutamate-5 …

   Promoter

   T7

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB168

   Plasmid

   #101671

   Purpose

   Opto-T7RNAP*(563-F1), equal expr.

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563-F1), equal expr.
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSW002-PpsbA-DsRed-Express2

   Plasmid

   #111257

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   DsRed-Express2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PpsbA

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Plasmid

   #176534

   Purpose

   Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associated IscB (Delaware Bay metagenome)
   

   Use

   Tags

   14xHis-MBP-bdSUMO and TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGT-Ah9

   Plasmid

   #122576

   Purpose

   MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   tetQ/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3521 pET15b Bid p15

   Plasmid

   #8782

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Use

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINE-3C-YFP

   Plasmid

   #214028

   Purpose

   Expression of fluorescent fusion proteins

   Depositor

   Ray Owens

   Insert

   YFP
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPINE-3C-mCherry

    Plasmid

    #214060

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    mCherry
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK234

    Plasmid

    #73324

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioC

    Depositor

    T. J. Kappock

    Inserts

    violacein biosynthesis protein VioA
    violacein biosynthesis protein VioB
    violacein biosynthesis protein VioC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 and n/a

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHS0806 His14-bdSUMO-AwaIscB

    Plasmid

    #176539

    Purpose

    Bacterial expression of His14-bdSUMO-AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Use

    CRISPR

    Tags

    14xHis-bdSUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMevB

    Plasmid

    #17819

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pDL52 (clone76)

    Plasmid

    #188579

    Purpose

    High-yield production of coenzyme F420 in E. coli

    Depositor

    Gerald Lackner

    Article

    Last et al Metab Eng. 2022 Jul 18. pii: S1096-7176(

    Inserts

    ribA
    ribD
    yigB
    fbiC
    cofC
    cofD
    cofI
    cofE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimization, synthetic ribosome binding si…

    Promoter

    T7 variants

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Use

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET empty polycistronic destination vector (2E)

    Plasmid

    #29775

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGC4593

    Plasmid

    #110206

    Purpose

    Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Inserts

    TEV protease
    aaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and Ptet

    Available since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid

    #214230

    Purpose

    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

    Depositor

    Neel Shah

    Article

    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

    Insert

    cSrc kinase SH2 domain (SRC Human)
    

    Use

    Tags

    6xHis, AviTag, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBP-P_cymR-PCymRC

    Plasmid

    #204071

    Purpose

    Type 0 promoter part

    Depositor

    Mikhail Shapiro

    Insert

    cymR repressor and PCymRC cumic acid-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge