32,838 results
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Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)Available SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB026a
Plasmid#109260PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPpsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);
ExpressionBacterialMutationamber codon at position 29 gIIIPromoterPpsp; PproKAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
5KCH (SETDB1)
Plasmid#101881PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (colE1 origin)
Plasmid#74092PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIB163
Plasmid#90186PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoterDepositorTypeEmpty backboneTagsHis-tag and Strep-tagExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT018e
Plasmid#107886PurposeR4 plasmid. Contains PBAD-EGFP-151TGA and is part of the CAMERA system for rewritable multi-event analog recording in bacteriaDepositorInsertPBAD-EGFP-151TGA
ExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ldhA
Plasmid#102288PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.DepositorInsertldhA gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP
Plasmid#108410PurposeMonomeric near-infrared fluorescent protein miRFP in bacterial expression plasmidDepositorInsertmiRFP
ExpressionBacterialPromoteraraBAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-Y-GECO2f
Plasmid#105065PurposeA yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterialDepositorInsertY-GECO2f
Tags6x His tagExpressionBacterialPromoterpBADAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
p4g3R
Plasmid#107958PurposeRFP reporter [PT7(-3G)-RBSB0034, low copy number]DepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET/LEGH
Plasmid#108226PurposeBacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tagDepositorInsertLBT-EGFP-His6
ExpressionBacterialPromoterT7, lacOAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-K10
Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_NTD1-80_E17R
Plasmid#104479Purposebacterial expression of TDP-43 NTD E17RDepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CSK-1/1
Plasmid#91542PurposeProtein expression and purification of human SH3 domain construct CSK-1/1DepositorInsertCSK-1/1 (CSK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABL1-1/1
Plasmid#91521PurposeProtein expression and purification of human SH3 domain construct ABL1-1/1DepositorInsertABL1-1/1 (ABL1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TP53BP2-1/1
Plasmid#91503PurposeProtein expression and purification of human SH3 domain construct TP53BP2-1/1DepositorInsertTP53BP2-1/1 (TP53BP2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3B-1/1
Plasmid#91462PurposeProtein expression and purification of human SH3 domain construct UBASH3B-1/1DepositorInsertUBASH3B-1/1 (UBASH3B Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCLS1-1/1
Plasmid#91437PurposeProtein expression and purification of human SH3 domain construct HCLS1-1/1DepositorInsertHCLS1-1/1 (HCLS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SHANK3-1/1
Plasmid#91421PurposeProtein expression and purification of human SH3 domain construct SHANK3-1/1DepositorInsertSHANK3-1/1 (SHANK3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BTK-1/1
Plasmid#91350PurposeProtein expression and purification of human SH3 domain construct BTK-1/1DepositorInsertBTK-1/1 (BTK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GAS7-1/1
Plasmid#91301PurposeProtein expression and purification of human SH3 domain construct GAS7-1/1DepositorInsertGAS7-1/1 (GAS7 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCK-1/1
Plasmid#91273PurposeProtein expression and purification of human SH3 domain construct HCK-1/1DepositorInsertHCK-1/1 (HCK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG93
Plasmid#105667Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 93 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 93PromoterpBad/arabinoseAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos4b-V69T
Plasmid#98572Purposephotoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpressionDepositorInsertmEos4b-V69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only