32,656 results
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Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARF6-His6(CO)
Plasmid#201887PurposeUsed for bacterial expression of human protein ARF6DepositorInsertARF6 (ARF6 Human)
Tags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Biomolecular FeedBack (FB)
Plasmid#109391PurposeThis construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.DepositorInsertpJ23100mut-dCas9-T- phtpG1-sgRNA
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEvoltac EcW-TGA-h9
Plasmid#218765Purposeencodes EcWRS-h9 and EcW-tRNA-TGADepositorInsertEcWRS-h9
ExpressionBacterialMutationS8A, V144S, V146APromotertacIAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-1
Plasmid#164858PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-1
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc-HA-GFP11
Plasmid#177726PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, and Mitochondrial Targeting SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJS96
Plasmid#207588PurposeGFP-TonBH20A expressionDepositorInsertGFP-TonBH20A
ExpressionBacterialMutationH20AAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJS65
Plasmid#207587PurposeGFP-TonB expressionDepositorInsertGFP-TonB
ExpressionBacterialAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
TagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsAvailable SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTA-HUH
Plasmid#221152PurposeHisG-URA3-HisG recycling type marker in pTAs for Yarrowia lipolyticaDepositorInsertHisG-URA3-HisG
ExpressionBacterial and YeastAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
BtuF
Plasmid#92209PurposeExpression of BtuF which is the vitamin B12 binding protein for BtuCD ABC TransporterDepositorInsertBtuF (btuF )
Tags6x His Tag and PelB Signal SequenceExpressionBacterialMutationBtuF signal sequence removed and uses the PelB si…PromoterT7 PromoterAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC059-HisMS2_PLP_NucPhos_pac
Plasmid#155039PurposeGeneration of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein geneDepositorInsertsMaturation Protein
Coat Protein Dimer
Nucleocapsid Phosphoprotein
ExpressionBacterialMutationRemove TypeIIs Restriction SitesPromoterT7Available SinceSept. 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Su(fu) (CT#99)
Plasmid#13856DepositorInsertSu(fu) (SUFU Chicken)
TagsGSTExpressionBacterialMutationNco I fragment from full length; ~2kbp- ATG to st…Available SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAJT194
Plasmid#202293PurposeType IIS cloning plasmid for LPattB5 integrations w/ OriTDepositorInsertsfGFP
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET15b-His-IkappaBalpha(FL)
Plasmid#44738DepositorAvailable SinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-S577A
Plasmid#92235PurposeExpress S577A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-S577A mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationSerine 577 to AlaninePromoterT7 promoterAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPS0379
Plasmid#115510PurposeSuitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFPDepositorInsertRlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQC
Plasmid#48100PurposeCumate-inducible expression of ECFP-tagged fusion proteinDepositorTypeEmpty backboneTags3xFLAG and ECFPExpressionBacterialPromoterPQ5Available SinceJan. 9, 2014AvailabilityAcademic Institutions and Nonprofits only