30,808 results

1. mKate-pBAD

   Plasmid

   #54826

   Purpose

   Localization: Protein Expression Vector , Excitation: 588, Emission: 635

   Depositor

   Dmitriy Chudakov, Michael Davidson

   Article

   Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

   Type

   Empty backbone

   Tags

   6xHis and mKate

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pGEX Syt1 C2AB (96-421)

   Plasmid

   #170643

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBP-ORF-xylE

   Plasmid

   #112780

   Purpose

   Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   xylE open reading frame
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCON1-ProB.gfp

   Plasmid

   #112516

   Purpose

   ProB driven gfpmut3 expression with synTT

   Depositor

   Olivia Steele-Mortimer

   Article

   Cooper et al Front Cell Infect Microbiol. 2017 Nov 16

   Insert

   ProB gfpmut3 TT
   

   Expression

   Bacterial

   Promoter

   ProB

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-hAIM2-His6

   Plasmid

   #51532

   Purpose

   Express His6-tagged hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Tags

   mcherry-2a

   Expression

   Bacterial

   Promoter

   neutrophil specific lyzC

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPbcsx28

   Plasmid

   #89909

   Purpose

   Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx28
   

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM cCol2 (CT#617)

   Plasmid

   #13943

   Depositor

   Cliff Tabin

   Article

   Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

   Insert

   Collagen II (COL2A1 Chicken)
   

   Expression

   Bacterial

   Mutation

   Nucleotides 182-616

   Available Since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. Salmonella MTAN

    Plasmid

    #64041

    Purpose

    Expression of S. enterica MTAN in E. coli

    Depositor

    Vern Schramm

    Article

    Hemeon et al Anal Chem. 2011 Jun 15;83(12):4996-5004.

    Insert

    Methylthioadenosine nucleosidase
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. USP5

    Plasmid

    #25299

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP5.001.835 (USP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PSIP1

    Plasmid

    #40744

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PSIP1 (PSIP1 )
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pET mCerulean LIC cloning vector (u-mCerulean)

    Plasmid

    #29773

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    mCerulean

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST14-DoubleCatcher-⍺-Lock

    Plasmid

    #216286

    Purpose

    Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ⍺-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST14-DoubleCatcher-β-Lock

    Plasmid

    #216287

    Purpose

    Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher β-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST14-DoubleCatcher-γ-Lock

    Plasmid

    #216288

    Purpose

    Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher γ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST14-DoubleCatcher-δ-Lock

    Plasmid

    #216289

    Purpose

    Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher δ-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST14-DoubleCatcher-ε-Lock

    Plasmid

    #216290

    Purpose

    Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    DoubleCatcher ε-Lock
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge