31,135 results

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K84M kinase-dead Nuak1-3xflag

Plasmid

#97219

Purpose

Plasmid expressing kinase-dead mutant Nuak1

Depositor

Huda Zoghbi

Article

Lasagna-Reeves et al Neuron. 2016 Oct 19;92(2):407-418. doi:

Insert

Nuak1 (NUAK1 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Kinase-dead K84M

Promoter

T7

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET mCerulean LIC cloning vector (u-mCerulean)

Plasmid

#29773

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCerulean

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/pT7 LasR

Plasmid

#193629

Purpose

Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasR

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJBL7005

Plasmid

#136944

Purpose

Malachite green aptamer with stability hairpin, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

Malachite green aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pKD227

Plasmid

#90954

Purpose

Expresses S. baltica TtrS under the Ptac promoter

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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2NVR

Plasmid

#51340

Purpose

Catalytic domain of human histone deacetylase HDAC7

Depositor

Cheryl Arrowsmith

Insert

HDAC7

Tags

His

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available Since

Feb. 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCoofy3

Plasmid

#43983

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-GST

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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AfNitB_pET29b

Plasmid

#215401

Purpose

Expression construct for AfNitB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AfNitB

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPPC005

Plasmid

#171140

Purpose

For integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

XylS-Pm

Use

CRISPR

Expression

Bacterial

Promoter

Pm

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pSA2

Plasmid

#52262

Purpose

Bacterial expression of human 6His-tagged SUMO2 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

Plasmid

#202023

Purpose

Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

Depositor

Mikhail Shapiro

Article

Hurt et al bioRxiv 2024.03.30.587094

Insert

Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)

Expression

Bacterial

Mutation

GvpA T6A-I48V

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherryGGBsMTS

Plasmid

#133625

Purpose

Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherryGGBsMTS

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-Nsp14-TEV-6His

Plasmid

#154952

Purpose

Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

Depositor

Justin Kollman

Insert

Nsp14-TEV-6His (ORF1ab SARS-CoV-2)

Tags

TEV-6His

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTYB2-eIF4B

Plasmid

#37226

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pH6HTC_IGF2BP3-Halo

Plasmid

#175310

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP3 (IGF2BP3 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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JMJD5

Plasmid

#39113

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD5 (KDM8 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
His-SUMO-hDDI2-D252N_pET11a

Plasmid

#169122

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pbacPuroR

Plasmid

#26348

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::PuroR::bacterial 3p flank

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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