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RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS

Plasmid

#244834

Purpose

Bacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpG Cas9

Use

CRISPR

Tags

10xHis-MBP and 2xSV40 NLS

Expression

Bacterial

Mutation

D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQE-80L 2xHis-Rab12 Q101L

Plasmid

#207347

Purpose

Bacterial expression of Rab12

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

Rab12 (RAB12 Human)

Tags

2xHis

Expression

Bacterial

Mutation

Q101L

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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spNW50

Plasmid

#173486

Purpose

Expresses spNW50 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW50

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRPN265NB-enh-MNase

Plasmid

#166035

Purpose

Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

Depositor

Marc Timmers

Article

Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

Insert

Glutathione-S-Transferase/enhancer/MNase

Tags

GST

Expression

Bacterial

Promoter

lac promoter / tac promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV LIC cloning vector (2T-T)

Plasmid

#29712

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-Thioredoxin-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pSGKP-spe

Plasmid

#117234

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-mitoTPNOX

Plasmid

#87854

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

mitoTPNOX

Tags

C-terminal FLAG tag with a linker and mitochondri…

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDXinit

Plasmid

#110101

Purpose

E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBAV1K-T5-gfp

Plasmid

#26702

Depositor

Ichiro Matsumura

Article

Bryksin et al PLoS One. 2010 . 5(10):e13244.

Insert

T5lacOlacOeGFP biobrick

Expression

Bacterial

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

Plasmid

#104394

Purpose

Yeast MCP-GFP expression

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

MCP-NLS-2xyeGFP

Expression

Bacterial and Yeast

Mutation

MCP fused to NLS(SV40) and 2x yeast codon optimiz…

Promoter

CYC1

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-CB2-HF

Plasmid

#121682

Purpose

Bacterial expression of cannabinoid receptor CB2 fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Calandra et al Biotechnology Letters

Insert

peripheral cannabinoid receptor (CNR2 Human)

Tags

His6 Flag tag

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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sfTq2ox-PBP5

Plasmid

#117960

Purpose

Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2ox-PBP5 (dacA )

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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p15aC-1D

Plasmid

#107863

Purpose

Bacterial expression of PIS

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

PIS

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 GST TEV LIC cloning vector (2G-T)

Plasmid

#29707

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, GST, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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