32,218 results
-
Plasmid#110065PurposeTrc1O promoter express gene in Synechocystis 6803DepositorTypeEmpty backboneTags3' end His tag and 5' end FLAG tagExpressionBacterialPromoterTrc1OAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only
-
NELFE-mOrange pHis-//
Plasmid#53303PurposeExpresses His-tagged NELF-E-mOrange fusion protein.DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEW4: pET-32a Dot1L(2-386)
Plasmid#124099PurposeDot1L(2-386) expression plasmidDepositorAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorT(sp)-mNeonGreen
Plasmid#205021PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorT-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
RPS6KA1
Plasmid#39021PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297AAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+FFlucWT
Plasmid#26158DepositorInsertFirefly luciferase
ExpressionBacterialMutationLacks the last 3 aa.Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm_P1T_DP-B1
Plasmid#90319PurposeIntegration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B1DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Stag-YCrs5
Plasmid#215163PurposeEncodes a yeast cadmium metallothionein fused with the S-tag at the N-terminusDepositorInsertStag-YCrs5
TagsS-tagExpressionBacterialPromoterT7 lacAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mSNX13-PX (558-677)
Plasmid#119094PurposeBacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)DepositorInsertmSNX13-PX (558-677)
TagsGSTExpressionBacterialPromotertacAvailable SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX14-PX (561-686)
Plasmid#119095PurposeBacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NUDT4 (NUDT4A-c001)
Plasmid#98241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T E7 Q
Plasmid#13648DepositorInsertHPV 16 E7 Q mutant (E7 Human)
TagsGSTExpressionBacterialMutationaa 30-37 changed from DSSEEEDE to QSSQQQQQ.Available SinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
B3-3
Plasmid#111473PurposeGlu-tRNA synthetaseDepositorInsertGlu-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFCiK5
Plasmid#167514PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.DepositorInsertCinRAM-mKelly2
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2
Plasmid#191751PurposeMicrotubule marker together with nuclear envelope marker driven by bidirectional promoterDepositorTagsGdp1 promoter, ToxA promoter, iRFP670, and mTagBF…ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 CS
Plasmid#8595DepositorAvailable SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN
Plasmid#221348PurposeExpress ChmN in E. coliDepositorInsertchmN
TagsHis6ExpressionBacterialAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pxyl-zapT-mScarlet-I
Plasmid#207249PurposeIntegrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoterDepositorInsertZapT-mScarlet-I
TagsmScarlet-IExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS6 AA 1035-1284
Plasmid#196904PurposeExpresses His-tagged Drosophila IntS6 amino acids 1035-1284DepositorAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry
Plasmid#187114PurposeProtein ExpressionDepositorAvailable SinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAT3
Plasmid#112589PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-GST fusionsDepositorTypeEmpty backboneTags6His-GSTExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Reb_82 aka PAS623
Plasmid#71228PurposeReb_1 with RebB(S82P, D88G)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebB(S82P, D88G), RebA (G53A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Reb_67 aka PAS627
Plasmid#71232PurposeReb_1 with RebA(S88A)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(G53A, S88A)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
TIF1A
Plasmid#74647PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianPromoter-Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGST TCF4 DBD
Plasmid#16488DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only