30,875 results

1. pDIV300

   Plasmid

   #204968

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMP12089

   Plasmid

   #222357

   Purpose

   Tn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO mTagBFP2

   Plasmid

   #68413

   Purpose

   TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mTagBFP2
   

   Use

   Gmap

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-UBE2S

   Plasmid

   #66714

   Purpose

   Expresses UBE2S (1-196) in E. coli

   Depositor

   David Komander

   Article

   Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

   Insert

   e2-epf (UBE2S )
   

   Tags

   GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCPP5951

   Plasmid

   #128719

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

   Insert

   hopR1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. HC25 pComb3xss anti-Homer1 nanobody

   Plasmid

   #134718

   Purpose

   Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
   

   Tags

   6xHis-HA

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SENP8 (full length))

   Plasmid

   #16361

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   human SENP8 full length (SENP8 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   human SENP8 full length, see Depositor Comments

   Available Since

   Dec. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSe_53

   Plasmid

   #109251

   Purpose

   pUC BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTK570

   Plasmid

   #110615

   Purpose

   BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   E2-Crimson
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET22-HT/spoR

    Plasmid

    #73631

    Purpose

    Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

    Depositor

    T. J. Kappock, James Williamson

    Article

    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

    Insert

    guanylate kinase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD061

    Plasmid

    #113315

    Purpose

    W108 scRNA.b1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA.b1_MS2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMS26

    Plasmid

    #32295

    Depositor

    Elisabeth Raleigh

    Article

    Sibley et al Nucleic Acids Res. 2012 Feb;40(3):e19. d

    Type

    Empty backbone

    Use

    Bacterial gene addition

    Expression

    Bacterial

    Promoter

    rhaB

    Available Since

    Sept. 26, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHS1184

    Plasmid

    #205965

    Purpose

    NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH
    

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29b-clbO

    Plasmid

    #114153

    Purpose

    Expresses ClbO

    Depositor

    Emily Balskus

    Article

    Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

    Insert

    clbO
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDSG319

    Plasmid

    #115596

    Purpose

    surface-displayed Ag3, used in composability experiments; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB19-PapRS#34-OmpA

    Plasmid

    #231105

    Purpose

    Vector for bacterial expression of a protein carrying the non-canonical amino acid p-(phenylazo)-L-phenylalanine (encoding the OmpA signal sequence for periplasmic secretion)

    Depositor

    Arne Skerra

    Article

    Mayrhofer et al Nat Commun. 2024 Dec 18;15(1):10693. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSR:MSMEG_5070:Dendra

    Plasmid

    #167375

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5070
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSR:MSMEG_5487:Dendra

    Plasmid

    #167440

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5487
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSR:MSMEG_5447:Dendra

    Plasmid

    #167431

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5447
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Promoter

    pmycTetO

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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