30,249 results

1. pJMP1335

   Plasmid

   #119269

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAH-CTX1-rhadCas9-native

   Plasmid

   #129392

   Purpose

   Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine, Histidine 840 to Alanine

   Promoter

   PrhaBAD

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6974 pPROEX-HTb-SSB1

   Plasmid

   #1232

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   ssb1 (SSB1 Budding Yeast)
   

   Use

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 9, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pSunS

   Plasmid

   #171672

   Purpose

   SunS expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6.MCV.LT206.V5(CM2B4)

   Plasmid

   #28190

   Depositor

   Patrick Moore

   Insert

   MCPyV genomic T antigen locus (206 wild-type strain)
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ana GvpC-SpyTag-His in pET28a

   Plasmid

   #85737

   Purpose

   Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (fused to SpyTag and His-tagged)
   

   Use

   Tags

   His-tag and SpyTag

   Expression

   Bacterial

   Mutation

   Added an extra glycine after the methionine at th…

   Promoter

   T7

   Available since

   March 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB085d

   Plasmid

   #79209

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ptet

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. NylB_pET29b

   Plasmid

   #215414

   Purpose

   Expression construct for NylB gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. S9_attL1-EGFP-C-term-attL2

    Plasmid

    #128473

    Purpose

    EGFP entry clone

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    EGFP entry clone
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1-H210Q

    Plasmid

    #15500

    Depositor

    Vance Lemmon

    Article

    Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

    Insert

    L1-H210Q (L1CAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Histidine 210 changed to Glutamine

    Promoter

    Available since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCDB364

    Plasmid

    #110275

    Purpose

    N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    10-His, TEV cleavage peptide, and mature DsbC (i.…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-CMV-MSI1(FL)

    Plasmid

    #60358

    Purpose

    express mouse MSI1 in mammalian cells

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (FL) (Msi1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Reb_79 aka PAS620

    Plasmid

    #71225

    Purpose

    Reb_1 with RebA(Q91R)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA( G53A, Q91R)

    Promoter

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

15. R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

    Plasmid

    #159541

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
    

    Use

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12D

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_BTF3-Halo

    Plasmid

    #175324

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYTK033

    Plasmid

    #65140

    Purpose

    Encodes Venus as a Type 3 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    Venus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTXB3-Flag PLK1

    Plasmid

    #68271

    Purpose

    bacterial expression of Plk1

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pGWF1

    Plasmid

    #18856

    Depositor

    Wolf Frommer

    Article

    Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

    Type

    Empty backbone

    Use

    Tags

    CFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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