30,880 results
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Plasmid#38850PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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pEB2-2ndVal Katushka
Plasmid#104029PurposePlasmid encoding 2ndVal KatushkaDepositorInsert2ndVal Katushka
UseLow copyExpressionBacterialMutationKatushka with valine inserted in 2nd positionPromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG84-pLas-TetRssrA
Plasmid#102455PurposeExpresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.DepositorAvailable SinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPA3
Plasmid#24777DepositorTypeEmpty backboneUseIntegrating vectorExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
ExpressionBacterialMutationall charged residues are changed to S/Q except fo…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
TagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
DYRK2
Plasmid#39031PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCORE
Plasmid#72231PurposeContains the CORE cassette with KlURA3 and KanMX4 markersDepositorInsertsKlURA3
kanMX4
ExpressionBacterialAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3
Plasmid#159700PurposeE. coli protein expressionDepositorAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2Available SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB14a-GFP
Plasmid#126117PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23119Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBY2_ligD(FnCpf1)
Plasmid#104623PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligDDepositorInsertcrRNA to ligD
UseCRISPRExpressionBacterialPromoterPj23119Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-TSC22D4
Plasmid#222671Purposeendogenously tag TSC22D4 with N-terminal mNeonDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST
Plasmid#203812PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5080
Plasmid#85106PurposePtrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expresDepositorInsertYFP-KaiC
ExpressionBacterialAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BASIC_11_RBS34-GFP-B0015
Plasmid#68145Purposestorage vector for BASIC part: RBS-GFP(ORF)DepositorInsertRBS-34-GFP-B0015
ExpressionBacterialAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only