34,359 results
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Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2-TetOminiCMV-OSK
Plasmid#136552PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
TagsHis6ExpressionBacterialPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-ProRS-His
Plasmid#124117PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET29b-H6_Streptavidin_sfGFP
Plasmid#124296PurposeHis6-tagged Streptavidin GFP fusion proteinDepositorInsertHis6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
TagsHis6 and T7 tagsExpressionBacterialAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE-Tn7-145
Plasmid#118561PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorExpressionBacterialAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorPublicationInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
TagsmCherryExpressionBacterial and YeastAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
TagsHis tagExpressionBacterialPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorTagsHis6ExpressionBacterialPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAD-LyseR
Plasmid#99244PurposeFor autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSAM_Cam
Plasmid#91570PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFC330
Plasmid#87842PurposeAMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection markerDepositorInsertsCas9
pyrG
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and native pro…Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
ExpressionBacterialPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDiGi
Plasmid#59323PurposeInducible expression of dsRed and GFP in bacteriaDepositorInsertsEGFP
dsRed
ExpressionBacterialPromoterPbad and PlacAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickExpressionBacterialPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
TagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by