30,091 results

1. spNW15

   Plasmid

   #173483

   Purpose

   Expresses spNW15 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW15
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTTCC

   Plasmid

   #164264

   Purpose

   Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

   Depositor

   Gudmundur Hreggvidsson

   Article

   Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

   Inserts

   Cas9
   gRNA scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcel and trc

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE60 I88A wgMDH

   Plasmid

   #204249

   Purpose

   Bacterial expression of I88A wgMDH with IPTG induction - Subunit interface mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   I88A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTXBI-mclover-YTHDC1-FL

   Plasmid

   #177121

   Purpose

   Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Tags

   Mxe GyrA intein-Chitin binding domain for protein…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His 3C MKK6 1-334

   Plasmid

   #87881

   Purpose

   E. coli expression of MKK6 aa 1-334

   Depositor

   Peter Mace

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   3C protease site and 6xHis

   Expression

   Bacterial

   Mutation

   aa 1-334

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAG87 FAST

   Plasmid

   #130719

   Purpose

   Expresses His-tagged FAST (also called YFAST) in bacteria

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   FAST
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFLP-hyg

   Plasmid

   #87831

   Purpose

   Expresses FLP recombinase with hyg selection marker

   Depositor

   Pep Charusanti

   Article

   Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

   Insert

   hyg
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   None

   Promoter

   heat-shock inducible

   Available since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniTurbo-His6_pET21a

   Plasmid

   #107178

   Purpose

   expresses His6-tagged miniTurbo in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWC2

   Plasmid

   #15960

   Depositor

   Jeff Gelles

   Article

   Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

   Insert

   Kinesin 1-401 BCCP his tag (Khc Fly, E. coli)
   

   Use

   Tags

   BCCP and His6

   Expression

   Bacterial

   Mutation

   amino acids 1-401

   Promoter

   Available since

   Nov. 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNHEJ-recX-sacB

    Plasmid

    #158718

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recX
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pmyc

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDT-SuperFi-Cas9

    Plasmid

    #183630

    Purpose

    Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

    Depositor

    David Taylor

    Article

    Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

    Promoter

    T7

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-AviTag-SnoopLigase

    Plasmid

    #105626

    Purpose

    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    AviTag-SnoopLigase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tol2-mpeg1-dendra2

    Plasmid

    #51462

    Purpose

    expresses photoconvertible dendra in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

    Insert

    mpeg-dendra
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mpeg1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet_huCAD_ICADL (TevS)

    Plasmid

    #100098

    Purpose

    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

    Depositor

    Jim Wells

    Article

    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

    Insert

    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two caspase cleavage sites (DETD-117 and DAVD-224…

    Promoter

    T7

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSET-mEosEM

    Plasmid

    #132708

    Purpose

    application in Correlative light-electron microscopy

    Depositor

    Pingyong Xu

    Article

    Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    mEosEM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ1996_v2

    Plasmid

    #140664

    Purpose

    dCas9 & Csy4

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    dCas9 & Csy4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge