30,803 results

1. pET-17b_Unc104-KIF5B-HALO

   Plasmid

   #169996

   Purpose

   Ultra processive kinesin KIF1A fused with KIF5B stalk for dimerization and HALO-tag for labeling

   Depositor

   Ron Vale

   Article

   Niekamp et al Nat Methods. 2020 Apr;17(4):437-441. doi

   Insert

   Kinesin-like protein Unc-104 fused with KIF5B
   

   Tags

   HALO-tag, 6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EKAR2G_design1_mTFP_175_Venus_173

   Plasmid

   #39830

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

3. 5787 pET28aSX104B/ pES42

   Plasmid

   #1229

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   Hsp104 (HSP104 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. ELF5_pet28a

   Plasmid

   #131659

   Purpose

   Bacterial expression of ETS gene ELF5

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF5 (ELF5 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HS-aPKC-CAAX-GFP

   Plasmid

   #105946

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   aPKC (Prkcz Rat)
   

   Tags

   CAAX and GFP

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 64 pCOLI_G2

   Plasmid

   #160206

   Purpose

   Gentamycin resistant polycistronic host vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMEH-T38M-750-luxAB-DAS+4

   Plasmid

   #49520

   Purpose

   Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   T38M-P750-luxAB-DAS+4
   

   Tags

   DAS+4 (AANDENYSENYADAS)

   Expression

   Bacterial

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBTD1

   Plasmid

   #25329

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MBTD1_5 (MBTD1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 130-566

   Available Since

   Aug. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pGEX-6P-1_WT RAD23A

   Plasmid

   #201456

   Purpose

   Expresses human RAD23A with an N-terminal GST tag in E. coli

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   tac promoter

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA528 SPASTIN , residues 108-200

    Plasmid

    #180628

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX2T VPS4B, residues 1-86

    Plasmid

    #180639

    Purpose

    Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    VPS4B (VPS4B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-86

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX C_K nsp1 CoV2

    Plasmid

    #175516

    Purpose

    Expresses C_K for bioconjugation on nsp1

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    C_K nsp1 CoV2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW115lac-hrpR

    Plasmid

    #61434

    Purpose

    pAPT110 encoding rbsH-hrpR-ter

    Depositor

    Martin Buck, Baojun Wang

    Article

    Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

    Insert

    rbsH-hrpR-ter
    

    Expression

    Bacterial

    Promoter

    pLac

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDJSVT89

    Plasmid

    #109303

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Tags

    TamA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Yes-SH2

    Plasmid

    #46532

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Yes (YES1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 152-261

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS4

    Plasmid

    #55751

    Purpose

    Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Expression

    Bacterial

    Mutation

    Residues 1-59 of KanR

    Promoter

    T7-lac

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgRNAkan-glpR

    Plasmid

    #169873

    Purpose

    Single guide RNA plasmid for Cas9 targeting glpR in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards glpR in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Reb_83 aka PAS624

    Plasmid

    #71229

    Purpose

    Reb_1 with RebA(G53A, D89G)

    Depositor

    Pamela Silver

    Article

    Polka et al ACS Synth Biol. 2016 Feb 3.

    Insert

    Reb locus
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    RebA(G53A, D89G)

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

19. GST TopBP1 C (aa 978-1522)

    Plasmid

    #20373

    Depositor

    Aziz Sancar

    Article

    Choi et al Nucleic Acids Res. 2009 Apr;37(5):1501-9

    Insert

    TopBP1 (978-1522) (TOPBP1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 978-1522

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_S100A11_S-100

    Plasmid

    #109804

    Purpose

    Protein expression and purification of S100A11_S-100

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    S100A11_S-100 (S100A11 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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