31,071 results
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Plasmid#176834PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert716
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB1k-GFP
Plasmid#35342DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSEVAT7Z1A
Plasmid#58300PurposeGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Orf9b
Plasmid#156485PurposeBacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tagDepositorInsertOrf9b
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC-TolB-NHSTIIT-GG
Plasmid#215792PurposeCloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a-LaM4-3xTS
Plasmid#162777PurposeBacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
TagsBAP, HA, His6, T7, and TS site from proCCKExpressionBacterialPromoterT7Available SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECJ3B
Plasmid#75466PurposeToggle switch plasmid containing lacI-pdt3BDepositorInsertlacI-pdt3B
ExpressionBacterialPromoterpLtetOAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB3033
Plasmid#113397PurposeTy3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducibleDepositorInsertTy3 Gag3 codon optimized for E. coli expression
TagsGag3-6XHis tagExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorAvailable SinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR)-DadR
Plasmid#191636PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementationDepositorInsertDadR
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZFYVE9C-c009
Plasmid#53618Purposebacterial expression plasmidDepositorInsertZFYVE9C
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Endophilin Full
Plasmid#47408DepositorInsertEndophilin
TagsGSTExpressionBacterialPromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
ExpressionBacterialPromoterrbcS-Tc: T7, chaperones: T12Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-VPS28
Plasmid#34803DepositorAvailable SinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(N)-SH2
Plasmid#46432DepositorInsertGap/RASGAP(N) (RASA1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 168-283PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEDJ400
Plasmid#177269PurposeUSER cloning-compatible 2μ vector (URA3)DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
RBM23_2xRRM_pGEX
Plasmid#135118PurposeRecombinant expression of RBM23 with dual-affinity tagDepositorAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only