33,539 results
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Plasmid#63816PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-A2-77p-Venus
Plasmid#64856PurposeExpresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tagDepositorInsertVWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
Tags7X His and VenusExpressionBacterialPromoterT7 PromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
ExpressionBacterialAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG 413
Plasmid#63859PurposeDetect translation initiation from CUG start codon (E. coli lacZ)DepositorAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Y203)
Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-Fab1 (S. aureus)
Plasmid#61691PurposeInducible expression of the FabI enzyme from S. aureusDepositorInsertFabI
TagsHisExpressionBacterialPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA4Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-A2NT
Plasmid#62257Purposeexpression of A2NT sgRNA from the arabinose-inducible promoterDepositorInsertA2NT sgRNA
UseCRISPRExpressionBacterialPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
ExpressionBacterialAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_27
Plasmid#63806Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
ExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXRU3
Plasmid#63152PurposeUra3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXRkMX
Plasmid#63147PurposeKan marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXRH3
Plasmid#63145PurposeHis3 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b-ITPA
Plasmid#60824Purposeto purify human ITPA from bacteriaDepositorAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
4TVR
Plasmid#61952PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUBH14 (UHRF2 Human)
TagsMHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAND_Int2
Plasmid#60590PurposeA transcriptional AND circuit constructed by layering NOR and NOT gatesDepositorInsertAND_Int2
ExpressionBacterialPromoterPPhlFAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAND_yfp
Plasmid#60592Purposeused with pSpec to generate an AND gate without memoryDepositorInsertAND_yfp
ExpressionBacterialAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only