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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-ThrRS

Plasmid

#124119

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Threonyl-tRNA ligase (thrS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Article

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Insert

c-Myc (MYC Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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IF-GFP-ATRX

Plasmid

#45444

Purpose

ATRX expression vector

Depositor

Michael Dyer

Article

Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

Insert

ATRX (ATRX Mouse, Human)

Use

Tags

GFP and HA

Expression

Bacterial and Mammalian

Mutation

isoform 2, missing codon E124

Promoter

PCMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a-LeuRS-His

Plasmid

#124113

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Leucyl-tRNA ligase (leuS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Mutation

Promoter

cspA

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis and TEV site

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-ProRS-His

Plasmid

#124117

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Prolyl-tRNA ligase (proS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-HisRS-His

Plasmid

#124111

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Histidyl-tRNA ligase (hisS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Article

Grotjohann et al elife. 2012;1:e00248. doi: 10.7554/eLife

Insert

rsEGFP2

Use

Tags

6xHisTag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pET28CLY1

Plasmid

#21760

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible one SGGSGG repeats

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pET21a-TyrRS-His

Plasmid

#124121

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tyrosyl-tRNA ligase (tyrS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Use

Tags

None

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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