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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Expression

Bacterial

Promoter

Rhamnose promoter

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSM41

Plasmid

#235728

Purpose

Protein expression of FL His6- human VPS34 in bacteria

Depositor

Roger Williams

Article

Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

Insert

VPS34 (PIK3C3 Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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Pediculus humanus corporis PINK1 (115 - 575)

Plasmid

#110750

Purpose

Bacterial expression of PhPINK1 (biochemistry)

Depositor

David Komander

Article

Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

Insert

Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)

Tags

N-His6-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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USP2

Plasmid

#36894

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP2 (USP2 Human)

Tags

His tag with thrombin cleavage

Expression

Bacterial

Available Since

June 19, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pGEX4T-1_WT_OCT4

Plasmid

#40633

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Tags

GST, TEV cleavage, and Thrombin cleavage

Expression

Bacterial

Promoter

tac

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pExp-Trx

Plasmid

#112577

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

8xHis-TrxA and Strep tag

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PKA-N-HiBiT

Plasmid

#211377

Purpose

Bacterially expressed HiBiT-PKA for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Human protein kinase cAMP-activated catalytic subunit alpha (PRKACA) transcript variant 1, with N-terminal HiBiT (PRKACA Human)

Tags

10xHis-tag with thrombin site and HiBiT Tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux and pTet

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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PB-EF1a-TetOn3G

Plasmid

#104543

Purpose

PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

Tet-On 3G

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSJX022

Plasmid

#232325

Purpose

Genome editing in Caulobacter crescentus

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and cat

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEvol-pAcFRS.1.t1

Plasmid

#73545

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.1.t1
pAcFRS.1.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSNK

Plasmid

#135193

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-SceI lamba Red

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

Plasmid

#122084

Purpose

Express M.EcoGII fused to TRF1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-TERF1

Use

Retroviral

Tags

V5 tag on Nter of TERF1

Expression

Bacterial and Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGRG25

Plasmid

#16665

Depositor

Nancy Craig

Article

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::MCS

Expression

Bacterial

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pET-Duet1_6xHis-TEV-eGFP-P62

Plasmid

#190929

Purpose

Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

P62 (SQSTM1 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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