33,480 results
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Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXD80RFP-pXD68Kan2-RFP
Plasmid#225329PurposeRSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistanceDepositorInsertRFP
ExpressionBacterialAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pColdI-DDX3_helicase_core
Plasmid#233618PurposeExpression of His-tagged helicase core region of DDX3X in E.coliDepositorAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSBLego
Plasmid#219967PurposeThe pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.DepositorTypeEmpty backboneTagsSLEHHHHHH and pelB signal sequenceExpressionBacterialPromoterpBADAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-Octo_VirD2_NLS-Cas9-6xHis
Plasmid#232296PurposeExpresses Cas9 protein fused with octopine-type VirD2-originating NLSDepositorInsertOcto_VirD2_NLS-Cas9-6xHis
UseCRISPRTags6xHis and Octopine-type VirD2-derived NLSExpressionBacterialPromoterT7Available sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCoofy1-psHaloTag1a
Plasmid#232940PurposePhotoswichable HaloTag: psHaloTag1a variantDepositorInsertphotoswitchable HaloTag 1a variant
TagsHis6x-HRV3CExpressionBacterialMutationE143W, A258W with linkers L1 (FAG) and L2 (P)PromoterT7Available sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_6IYE
Plasmid#234237PurposeHeterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coliDepositorInsert6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3KB5
Plasmid#234225PurposeHeterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coliDepositorAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2PPN
Plasmid#234190PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coliDepositorAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1UXZ
Plasmid#234166PurposeHeterologous protein expression of cellulase B in Escherichia coliDepositorInsert1UXZ
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSR1154
Plasmid#221653PurposeTetracycline sensor v3 for Staphylococcus aureusDepositorInsertssfGFP cassette
TetR cassette
UseSynthetic BiologyExpressionBacterialPromoterPXyl-TetO and PZwfAvailable sinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
esmGFP-C10-pVTCB052
Plasmid#231303PurposePlasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coliDepositorInsertesmGFP-C10
ExpressionBacterialAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCV006-ermg
Plasmid#232824PurposeRandom transposon mutagenesis vector for Bacteroidales species - erythromycinDepositorInsertermG
ExpressionBacterialAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH81 pET21b Rat1-His
Plasmid#231650PurposeBacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tagDepositorAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pZH82 pET28a-Rai1
Plasmid#231651PurposeBacterial expression vector expressing yeast S. cerevisiae rai1, no tagDepositorInsertrai1 (RAI1 Budding Yeast)
ExpressionBacterialAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-RFP amber
Plasmid#229524PurposemCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13DepositorPublicationInsertRFP amber
ExpressionBacterialPromoterT7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only